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Prevalence of HPV 16 and HPV 18 lineages in Galicia, Spain.

Pérez S, Cid A, Iñarrea A, Pato M, Lamas MJ, Couso B, Gil M, Alvarez MJ, Rey S, López-Miragaya I, Melón S, Oña Md - PLoS ONE (2014)

Bottom Line: In conclusion, lineages A of HPV16/18 were predominant in Spain.Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A.Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.

ABSTRACT
Genetic variants of human papillomavirus types 16 and 18 (HPV16/18) could differ in their cancer risk. We studied the prevalence and association with high-grade cervical lesions of different HPV16/18 variant lineages in a case-control study including 217 cases (cervical intraepithelial neoplasia grade 2 or grade 3 or worse: CIN2 or CIN3+) and 116 controls (no CIN2 or CIN3+ in two-year follow-up). HPV lineages were determined by sequencing the long control region (LCR) and the E6 gene. Phylogenetic analysis of HPV16 confirmed that isolates clustered into previously described lineages: A (260, 87.5%), B (4, 1.3%), C (8, 2.7%), and D (25, 8.4%). Lineage D/lineage A strains were, respectively, detected in 4/82 control patients, 19/126 CIN3+ cases (OR = 3.1, 95%CI: 1.0-12.9, p = 0.04), 6/1 glandular high-grade lesions (OR = 123, 95%CI: 9.7-5713.6, p<0.0001), and 4/5 invasive lesions (OR = 16.4, 95%CI: 2.2-113.7, p = 0.002). HPV18 clustered in lineages A (32, 88.9%) and B (4, 11.1%). Lineage B/lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4-565.4, p = 0.12). In conclusion, lineages A of HPV16/18 were predominant in Spain. Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A. Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant. Large well-designed studies are needed before the application of HPV lineage detection in clinical settings.

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Phylogenetic tree of the HPV18 isolates based on LCR/E6 sequences.Phylogenetic analysis confirmed the presence of two lineages [4]: A and B. A maximum likelihood (ML) tree was inferred from an alignment of nine reference sequences and seven study sequences of HPV18 LCR-E6 using RAxML HPC v8 [22]. Highly related sequences (<0.4 differences) from the study were not included in this figure. Reference sequences were denominated as lineage/strain.
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pone-0104678-g005: Phylogenetic tree of the HPV18 isolates based on LCR/E6 sequences.Phylogenetic analysis confirmed the presence of two lineages [4]: A and B. A maximum likelihood (ML) tree was inferred from an alignment of nine reference sequences and seven study sequences of HPV18 LCR-E6 using RAxML HPC v8 [22]. Highly related sequences (<0.4 differences) from the study were not included in this figure. Reference sequences were denominated as lineage/strain.

Mentions: Two lineages previously described of HPV18 were found in the studied population (Fig. 4 and 5). HPV18 clustered in lineage A (32/36, 88.9%) and lineage B (4/36, 11.1%) (table 3). Lineage B and lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4–565.4, p = 0.12, control group as reference) (table 3).


Prevalence of HPV 16 and HPV 18 lineages in Galicia, Spain.

Pérez S, Cid A, Iñarrea A, Pato M, Lamas MJ, Couso B, Gil M, Alvarez MJ, Rey S, López-Miragaya I, Melón S, Oña Md - PLoS ONE (2014)

Phylogenetic tree of the HPV18 isolates based on LCR/E6 sequences.Phylogenetic analysis confirmed the presence of two lineages [4]: A and B. A maximum likelihood (ML) tree was inferred from an alignment of nine reference sequences and seven study sequences of HPV18 LCR-E6 using RAxML HPC v8 [22]. Highly related sequences (<0.4 differences) from the study were not included in this figure. Reference sequences were denominated as lineage/strain.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128731&req=5

pone-0104678-g005: Phylogenetic tree of the HPV18 isolates based on LCR/E6 sequences.Phylogenetic analysis confirmed the presence of two lineages [4]: A and B. A maximum likelihood (ML) tree was inferred from an alignment of nine reference sequences and seven study sequences of HPV18 LCR-E6 using RAxML HPC v8 [22]. Highly related sequences (<0.4 differences) from the study were not included in this figure. Reference sequences were denominated as lineage/strain.
Mentions: Two lineages previously described of HPV18 were found in the studied population (Fig. 4 and 5). HPV18 clustered in lineage A (32/36, 88.9%) and lineage B (4/36, 11.1%) (table 3). Lineage B and lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4–565.4, p = 0.12, control group as reference) (table 3).

Bottom Line: In conclusion, lineages A of HPV16/18 were predominant in Spain.Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A.Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.

ABSTRACT
Genetic variants of human papillomavirus types 16 and 18 (HPV16/18) could differ in their cancer risk. We studied the prevalence and association with high-grade cervical lesions of different HPV16/18 variant lineages in a case-control study including 217 cases (cervical intraepithelial neoplasia grade 2 or grade 3 or worse: CIN2 or CIN3+) and 116 controls (no CIN2 or CIN3+ in two-year follow-up). HPV lineages were determined by sequencing the long control region (LCR) and the E6 gene. Phylogenetic analysis of HPV16 confirmed that isolates clustered into previously described lineages: A (260, 87.5%), B (4, 1.3%), C (8, 2.7%), and D (25, 8.4%). Lineage D/lineage A strains were, respectively, detected in 4/82 control patients, 19/126 CIN3+ cases (OR = 3.1, 95%CI: 1.0-12.9, p = 0.04), 6/1 glandular high-grade lesions (OR = 123, 95%CI: 9.7-5713.6, p<0.0001), and 4/5 invasive lesions (OR = 16.4, 95%CI: 2.2-113.7, p = 0.002). HPV18 clustered in lineages A (32, 88.9%) and B (4, 11.1%). Lineage B/lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4-565.4, p = 0.12). In conclusion, lineages A of HPV16/18 were predominant in Spain. Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A. Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant. Large well-designed studies are needed before the application of HPV lineage detection in clinical settings.

Show MeSH
Related in: MedlinePlus