Altered expression of diabetes-related genes in Alzheimer's disease brains: the Hisayama study.
Bottom Line: Relevant changes in gene expression identified by microarray analysis were validated by quantitative real-time reverse-transcription polymerase chain reaction and western blotting.Genes involved in noninsulin-dependent DM and obesity were significantly altered in both AD brains and the AD mouse model, as were genes related to psychiatric disorders and AD.These results indicate that altered expression of genes related to DM in AD brains is a result of AD pathology, which may thereby be exacerbated by peripheral insulin resistance or DM.
Affiliation: Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Department of Neurosurgery, Graduate School of Medical Sciences.Show MeSH
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Mentions: We then examined expression of PCSK1 protein in mouse brain by laser scanning immunofluorescence microscopy (Fig. 5). In 15-month-old male non-Tg brains, we detected PCSK1 expression in most neurons in the cerebral cortex and hippocampus (Fig. 5A,B). In non-Tg hippocampus, PCSK1 expression is prominent in CA3 and CA2 subregions and to a lesser extent in CA1 and the dentate gyrus (DG) (Fig. 5C). We found that expression level of PCSK1 was significantly diminished in 3xTg-AD-H brains, including in the cerebral cortex (Fig. 5A) and hippocampus (Fig. 5B,C), as confirmed by microarray data.Figure 5.
Affiliation: Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Department of Neurosurgery, Graduate School of Medical Sciences.