Limits...
Altered expression of diabetes-related genes in Alzheimer's disease brains: the Hisayama study.

Hokama M, Oka S, Leon J, Ninomiya T, Honda H, Sasaki K, Iwaki T, Ohara T, Sasaki T, LaFerla FM, Kiyohara Y, Nakabeppu Y - Cereb. Cortex (2013)

Bottom Line: Relevant changes in gene expression identified by microarray analysis were validated by quantitative real-time reverse-transcription polymerase chain reaction and western blotting.Genes involved in noninsulin-dependent DM and obesity were significantly altered in both AD brains and the AD mouse model, as were genes related to psychiatric disorders and AD.These results indicate that altered expression of genes related to DM in AD brains is a result of AD pathology, which may thereby be exacerbated by peripheral insulin resistance or DM.

View Article: PubMed Central - PubMed

Affiliation: Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Department of Neurosurgery, Graduate School of Medical Sciences.

Show MeSH

Related in: MedlinePlus

Evaluation of PCSK1 protein levels in mouse brain by laser scanning immunofluorescence confocal microscopy. (A) PCSK1 expression in the cerebral cortex. (B) PCSK1 expression in the hippocampus. (C) Magnified images of the hippocampal subregions CA1, CA2, CA3, and DG. Brain sections were prepared from 15-month-old non-Tg and 3x-Tg-AD-H male mice. Sections were reacted with anti-PCSK1 antibody (green) and an anti-NeuN antibody (red), and nuclear DNA was counterstained with DAPI (blue). Scale bars: A, B, 100 μm; C, 20 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4128707&req=5

BHT101F5: Evaluation of PCSK1 protein levels in mouse brain by laser scanning immunofluorescence confocal microscopy. (A) PCSK1 expression in the cerebral cortex. (B) PCSK1 expression in the hippocampus. (C) Magnified images of the hippocampal subregions CA1, CA2, CA3, and DG. Brain sections were prepared from 15-month-old non-Tg and 3x-Tg-AD-H male mice. Sections were reacted with anti-PCSK1 antibody (green) and an anti-NeuN antibody (red), and nuclear DNA was counterstained with DAPI (blue). Scale bars: A, B, 100 μm; C, 20 μm.

Mentions: We then examined expression of PCSK1 protein in mouse brain by laser scanning immunofluorescence microscopy (Fig. 5). In 15-month-old male non-Tg brains, we detected PCSK1 expression in most neurons in the cerebral cortex and hippocampus (Fig. 5A,B). In non-Tg hippocampus, PCSK1 expression is prominent in CA3 and CA2 subregions and to a lesser extent in CA1 and the dentate gyrus (DG) (Fig. 5C). We found that expression level of PCSK1 was significantly diminished in 3xTg-AD-H brains, including in the cerebral cortex (Fig. 5A) and hippocampus (Fig. 5B,C), as confirmed by microarray data.Figure 5.


Altered expression of diabetes-related genes in Alzheimer's disease brains: the Hisayama study.

Hokama M, Oka S, Leon J, Ninomiya T, Honda H, Sasaki K, Iwaki T, Ohara T, Sasaki T, LaFerla FM, Kiyohara Y, Nakabeppu Y - Cereb. Cortex (2013)

Evaluation of PCSK1 protein levels in mouse brain by laser scanning immunofluorescence confocal microscopy. (A) PCSK1 expression in the cerebral cortex. (B) PCSK1 expression in the hippocampus. (C) Magnified images of the hippocampal subregions CA1, CA2, CA3, and DG. Brain sections were prepared from 15-month-old non-Tg and 3x-Tg-AD-H male mice. Sections were reacted with anti-PCSK1 antibody (green) and an anti-NeuN antibody (red), and nuclear DNA was counterstained with DAPI (blue). Scale bars: A, B, 100 μm; C, 20 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128707&req=5

BHT101F5: Evaluation of PCSK1 protein levels in mouse brain by laser scanning immunofluorescence confocal microscopy. (A) PCSK1 expression in the cerebral cortex. (B) PCSK1 expression in the hippocampus. (C) Magnified images of the hippocampal subregions CA1, CA2, CA3, and DG. Brain sections were prepared from 15-month-old non-Tg and 3x-Tg-AD-H male mice. Sections were reacted with anti-PCSK1 antibody (green) and an anti-NeuN antibody (red), and nuclear DNA was counterstained with DAPI (blue). Scale bars: A, B, 100 μm; C, 20 μm.
Mentions: We then examined expression of PCSK1 protein in mouse brain by laser scanning immunofluorescence microscopy (Fig. 5). In 15-month-old male non-Tg brains, we detected PCSK1 expression in most neurons in the cerebral cortex and hippocampus (Fig. 5A,B). In non-Tg hippocampus, PCSK1 expression is prominent in CA3 and CA2 subregions and to a lesser extent in CA1 and the dentate gyrus (DG) (Fig. 5C). We found that expression level of PCSK1 was significantly diminished in 3xTg-AD-H brains, including in the cerebral cortex (Fig. 5A) and hippocampus (Fig. 5B,C), as confirmed by microarray data.Figure 5.

Bottom Line: Relevant changes in gene expression identified by microarray analysis were validated by quantitative real-time reverse-transcription polymerase chain reaction and western blotting.Genes involved in noninsulin-dependent DM and obesity were significantly altered in both AD brains and the AD mouse model, as were genes related to psychiatric disorders and AD.These results indicate that altered expression of genes related to DM in AD brains is a result of AD pathology, which may thereby be exacerbated by peripheral insulin resistance or DM.

View Article: PubMed Central - PubMed

Affiliation: Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Department of Neurosurgery, Graduate School of Medical Sciences.

Show MeSH
Related in: MedlinePlus