Limits...
Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model.

Chirakul S, Bartpho T, Wongsurawat T, Taweechaisupapong S, Karoonutaisiri N, Talaat AM, Wongratanacheewin S, Ernst RK, Sermswan RW - PLoS ONE (2014)

Bottom Line: Severity of the disease is thought to be dependent on both the health status of the host, including diabetes mellitus and kidney disease, and bacterial-derived factors.One striking observation was the increased expression of BPSS1520 (bprC), located downstream of bprD, in the bprD mutant.BprC is a regulator of T6SS-1 that is required for the virulence of B. pseudomallei in murine infection models.

View Article: PubMed Central - PubMed

Affiliation: Melioidosis Research Center, Khon Kaen University, Khon Kaen, Thailand; Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

ABSTRACT
The Gram-negative saprophytic bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a severe infectious disease of both humans and animals. Severity of the disease is thought to be dependent on both the health status of the host, including diabetes mellitus and kidney disease, and bacterial-derived factors. To identify the bacterial factors important during an acute infection, gene expression profiles in the spleen, lung, and liver of BALB/c (Th2 prototype) and C57BL/6 mice (Th1 prototype) were determined using DNA microarrays. This analysis identified BPSS1521 (bprD), a predicted transcriptional regulator located in the type III secretion system (T3SS-3) operon, to be up regulated, specifically in C57BL/6 mice. BALB/c mice infected with a bprD mutant showed a shorter time to death and increased inflammation, as determined by histopathological analysis and enumeration of bacteria in the spleen. Elevated numbers of multinucleated giant cells (MNGCs), which is the hallmark of melioidosis, were detected in both the wild-type and the bprD mutants; a similar elevation occurs in melioidosis patients. One striking observation was the increased expression of BPSS1520 (bprC), located downstream of bprD, in the bprD mutant. BprC is a regulator of T6SS-1 that is required for the virulence of B. pseudomallei in murine infection models. Deletion of bprD led to the overexpression of bprC and a decreased time to death. bprD expression was elevated in C57BL/6--as compared to BALB/c--mice, suggesting a role for BprD in the natural resistance of C57BL/6 mice to B. pseudomallei. Ultimately, this analysis using mice with different immune backgrounds may enhance our understanding of the outcomes of infection in a variety of models.

Show MeSH

Related in: MedlinePlus

Venn diagrams showing the number of genes differentially expressed in each organ.Genes expressed at higher levels in the spleen (pink); lung (green); and liver (yellow) of BALB/C (A) and C57BL/6 (B) mice compared to in vitro.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4128674&req=5

pone-0104313-g001: Venn diagrams showing the number of genes differentially expressed in each organ.Genes expressed at higher levels in the spleen (pink); lung (green); and liver (yellow) of BALB/C (A) and C57BL/6 (B) mice compared to in vitro.

Mentions: To reduce the complexity of this dataset, genes whose expression was increased in all three of the target organs (lungs, liver, and spleen) were selected initially. This analysis resulted in: 1) 42 genes in BALB/c mice (Figure 1A); 2) 49 genes in C57BL/6 mice (Figure 1B); and 3) 34 genes whose expression was increased in both mouse strains. The majority of the genes in these three datasets were metabolic or housekeeping genes.


Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model.

Chirakul S, Bartpho T, Wongsurawat T, Taweechaisupapong S, Karoonutaisiri N, Talaat AM, Wongratanacheewin S, Ernst RK, Sermswan RW - PLoS ONE (2014)

Venn diagrams showing the number of genes differentially expressed in each organ.Genes expressed at higher levels in the spleen (pink); lung (green); and liver (yellow) of BALB/C (A) and C57BL/6 (B) mice compared to in vitro.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128674&req=5

pone-0104313-g001: Venn diagrams showing the number of genes differentially expressed in each organ.Genes expressed at higher levels in the spleen (pink); lung (green); and liver (yellow) of BALB/C (A) and C57BL/6 (B) mice compared to in vitro.
Mentions: To reduce the complexity of this dataset, genes whose expression was increased in all three of the target organs (lungs, liver, and spleen) were selected initially. This analysis resulted in: 1) 42 genes in BALB/c mice (Figure 1A); 2) 49 genes in C57BL/6 mice (Figure 1B); and 3) 34 genes whose expression was increased in both mouse strains. The majority of the genes in these three datasets were metabolic or housekeeping genes.

Bottom Line: Severity of the disease is thought to be dependent on both the health status of the host, including diabetes mellitus and kidney disease, and bacterial-derived factors.One striking observation was the increased expression of BPSS1520 (bprC), located downstream of bprD, in the bprD mutant.BprC is a regulator of T6SS-1 that is required for the virulence of B. pseudomallei in murine infection models.

View Article: PubMed Central - PubMed

Affiliation: Melioidosis Research Center, Khon Kaen University, Khon Kaen, Thailand; Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

ABSTRACT
The Gram-negative saprophytic bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a severe infectious disease of both humans and animals. Severity of the disease is thought to be dependent on both the health status of the host, including diabetes mellitus and kidney disease, and bacterial-derived factors. To identify the bacterial factors important during an acute infection, gene expression profiles in the spleen, lung, and liver of BALB/c (Th2 prototype) and C57BL/6 mice (Th1 prototype) were determined using DNA microarrays. This analysis identified BPSS1521 (bprD), a predicted transcriptional regulator located in the type III secretion system (T3SS-3) operon, to be up regulated, specifically in C57BL/6 mice. BALB/c mice infected with a bprD mutant showed a shorter time to death and increased inflammation, as determined by histopathological analysis and enumeration of bacteria in the spleen. Elevated numbers of multinucleated giant cells (MNGCs), which is the hallmark of melioidosis, were detected in both the wild-type and the bprD mutants; a similar elevation occurs in melioidosis patients. One striking observation was the increased expression of BPSS1520 (bprC), located downstream of bprD, in the bprD mutant. BprC is a regulator of T6SS-1 that is required for the virulence of B. pseudomallei in murine infection models. Deletion of bprD led to the overexpression of bprC and a decreased time to death. bprD expression was elevated in C57BL/6--as compared to BALB/c--mice, suggesting a role for BprD in the natural resistance of C57BL/6 mice to B. pseudomallei. Ultimately, this analysis using mice with different immune backgrounds may enhance our understanding of the outcomes of infection in a variety of models.

Show MeSH
Related in: MedlinePlus