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Dynamic remodeling of microbial biofilms by functionally distinct exopolysaccharides.

Chew SC, Kundukad B, Seviour T, van der Maarel JR, Yang L, Rice SA, Doyle P, Kjelleberg S - MBio (2014)

Bottom Line: Conversely, Pel reduced effective cross-linking within the matrix.The wild-type biofilm decreased in effective cross-linking over time, which would be advantageous for the spreading and colonization of new surfaces.The exopolysaccharides were also found to have profound effects on the spatial organization and integration of P. aeruginosa in a mixed-species biofilm model of P. aeruginosa-Staphylococcus aureus.

View Article: PubMed Central - PubMed

Affiliation: BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, National University of Singapore, Singapore.

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Related in: MedlinePlus

Confocal images of the various EPS mutants in the biofilm streamer cultivation system. Flow direction is horizontal (left to right) and parallel to the steel mesh. (A) Alg+ Pel+ Psl+ cells initially forming rough surface-attached biofilms that become smooth with enhanced spreading. (B) Alg+ Pel− Psl+ cells forming rough surface-attached biofilms that develop large microcolonies with minimal spreading. (C) Alg+ Pel+ Psl− cells forming smooth biofilms with extensive streamer formation that extends across the mesh. (D) Alg+ Pel− Psl− cells do not form biofilm. Insets show enlarged views of the biofilms.
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fig6: Confocal images of the various EPS mutants in the biofilm streamer cultivation system. Flow direction is horizontal (left to right) and parallel to the steel mesh. (A) Alg+ Pel+ Psl+ cells initially forming rough surface-attached biofilms that become smooth with enhanced spreading. (B) Alg+ Pel− Psl+ cells forming rough surface-attached biofilms that develop large microcolonies with minimal spreading. (C) Alg+ Pel+ Psl− cells forming smooth biofilms with extensive streamer formation that extends across the mesh. (D) Alg+ Pel− Psl− cells do not form biofilm. Insets show enlarged views of the biofilms.

Mentions: Elastic materials reversibly deform under shear, whereas viscous materials irreversibly deform under shear. To test the hypothesis that Psl makes biofilms more elastic and thus resistant to deformation shear forces, whereas Pel makes the biofilm more malleable, a biofilm streamer cultivation system, which involved growing biofilms on a steel mesh, was developed to assess the correlation between the various P. aeruginosa EPS mutants and biofilm streamer morphology. During the cultivation of P. aeruginosa EPS mutant biofilms, Alg+ Pel+ Psl+ cells initially formed rough surface-attached biofilms. In the later stages, the biofilms became smooth with enhanced spreading (Fig. 6A). Alg+ Pel− Psl+ cells formed rough surface-attached biofilms that developed tall microcolonies with minimal spreading (Fig. 6B). Alg+ Pel+ Psl− cells formed smooth biofilms with extensive streamer formation. In the later stages, the steel mesh was completely covered by the biofilms (Fig. 6C). Alg+ Pel− Psl− cells attached to the mesh but could not form a biofilm (Fig. 6D).


Dynamic remodeling of microbial biofilms by functionally distinct exopolysaccharides.

Chew SC, Kundukad B, Seviour T, van der Maarel JR, Yang L, Rice SA, Doyle P, Kjelleberg S - MBio (2014)

Confocal images of the various EPS mutants in the biofilm streamer cultivation system. Flow direction is horizontal (left to right) and parallel to the steel mesh. (A) Alg+ Pel+ Psl+ cells initially forming rough surface-attached biofilms that become smooth with enhanced spreading. (B) Alg+ Pel− Psl+ cells forming rough surface-attached biofilms that develop large microcolonies with minimal spreading. (C) Alg+ Pel+ Psl− cells forming smooth biofilms with extensive streamer formation that extends across the mesh. (D) Alg+ Pel− Psl− cells do not form biofilm. Insets show enlarged views of the biofilms.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128364&req=5

fig6: Confocal images of the various EPS mutants in the biofilm streamer cultivation system. Flow direction is horizontal (left to right) and parallel to the steel mesh. (A) Alg+ Pel+ Psl+ cells initially forming rough surface-attached biofilms that become smooth with enhanced spreading. (B) Alg+ Pel− Psl+ cells forming rough surface-attached biofilms that develop large microcolonies with minimal spreading. (C) Alg+ Pel+ Psl− cells forming smooth biofilms with extensive streamer formation that extends across the mesh. (D) Alg+ Pel− Psl− cells do not form biofilm. Insets show enlarged views of the biofilms.
Mentions: Elastic materials reversibly deform under shear, whereas viscous materials irreversibly deform under shear. To test the hypothesis that Psl makes biofilms more elastic and thus resistant to deformation shear forces, whereas Pel makes the biofilm more malleable, a biofilm streamer cultivation system, which involved growing biofilms on a steel mesh, was developed to assess the correlation between the various P. aeruginosa EPS mutants and biofilm streamer morphology. During the cultivation of P. aeruginosa EPS mutant biofilms, Alg+ Pel+ Psl+ cells initially formed rough surface-attached biofilms. In the later stages, the biofilms became smooth with enhanced spreading (Fig. 6A). Alg+ Pel− Psl+ cells formed rough surface-attached biofilms that developed tall microcolonies with minimal spreading (Fig. 6B). Alg+ Pel+ Psl− cells formed smooth biofilms with extensive streamer formation. In the later stages, the steel mesh was completely covered by the biofilms (Fig. 6C). Alg+ Pel− Psl− cells attached to the mesh but could not form a biofilm (Fig. 6D).

Bottom Line: Conversely, Pel reduced effective cross-linking within the matrix.The wild-type biofilm decreased in effective cross-linking over time, which would be advantageous for the spreading and colonization of new surfaces.The exopolysaccharides were also found to have profound effects on the spatial organization and integration of P. aeruginosa in a mixed-species biofilm model of P. aeruginosa-Staphylococcus aureus.

View Article: PubMed Central - PubMed

Affiliation: BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, National University of Singapore, Singapore.

Show MeSH
Related in: MedlinePlus