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Dynamic remodeling of microbial biofilms by functionally distinct exopolysaccharides.

Chew SC, Kundukad B, Seviour T, van der Maarel JR, Yang L, Rice SA, Doyle P, Kjelleberg S - MBio (2014)

Bottom Line: Conversely, Pel reduced effective cross-linking within the matrix.The wild-type biofilm decreased in effective cross-linking over time, which would be advantageous for the spreading and colonization of new surfaces.The exopolysaccharides were also found to have profound effects on the spatial organization and integration of P. aeruginosa in a mixed-species biofilm model of P. aeruginosa-Staphylococcus aureus.

View Article: PubMed Central - PubMed

Affiliation: BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, National University of Singapore, Singapore.

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Related in: MedlinePlus

Ratios of various types of particles (z axis) in biofilm-forming strains (x axis) at days 4 and 5 after continuous feeding with particles upon inoculation of the flow cell. All particle numbers (y axis) are standardized to 100 particles of a size of 1.0 µm and carboxylate modification. (A) Ratio of carboxylate particles according to sizes of 1.0 (purple), 0.5 (red), and 0.2 (orange) μm. (B) Ratio of 1.0-µm particles according to carboxylate (purple) and sulfate (blue) surface modification.
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fig2: Ratios of various types of particles (z axis) in biofilm-forming strains (x axis) at days 4 and 5 after continuous feeding with particles upon inoculation of the flow cell. All particle numbers (y axis) are standardized to 100 particles of a size of 1.0 µm and carboxylate modification. (A) Ratio of carboxylate particles according to sizes of 1.0 (purple), 0.5 (red), and 0.2 (orange) μm. (B) Ratio of 1.0-µm particles according to carboxylate (purple) and sulfate (blue) surface modification.

Mentions: Particles were incorporated from the medium into the three biofilm-forming strains (Alg+ Pel+ Psl+, Alg+ Pel− Psl+, and Alg+ Pel+ Psl−), with increased uptake of the larger particles relative to the smaller particles. Particles were counted in 4- and 5-day biofilms, and the ratio of 1.0- to 0.5- and 0.2-µm particles was used as a measure of size selectivity in the three biofilm-forming strains (Fig. 2A). The Alg+ Pel+ Psl+ biofilm had 1.0- to 0.5- and 0.2-µm particle ratios of 2.02 ± 0.41 and 4.13 ± 1.24, respectively, while Alg+ Pel− Psl+ biofilm displayed ratios of 1.85 ± 0.55 and 4.81 ± 1.12, respectively. The Alg+ Pel+ Psl− biofilm, which was significantly impaired in biofilm development, had lower 1.0- to 0.5- and 0.2-µm particle ratios of 1.52 ± 0.31 and 2.44 ± 0.73, respectively. It was observed that the preference for 1.0-µm particles over the smaller particles was predominant in the microcolonies (Fig. 3). Hence strains that expressed the Psl polysaccharide and that formed more differentiated biofilms gave higher ratios of 1.0- to 0.5- and 0.2-µm particles.


Dynamic remodeling of microbial biofilms by functionally distinct exopolysaccharides.

Chew SC, Kundukad B, Seviour T, van der Maarel JR, Yang L, Rice SA, Doyle P, Kjelleberg S - MBio (2014)

Ratios of various types of particles (z axis) in biofilm-forming strains (x axis) at days 4 and 5 after continuous feeding with particles upon inoculation of the flow cell. All particle numbers (y axis) are standardized to 100 particles of a size of 1.0 µm and carboxylate modification. (A) Ratio of carboxylate particles according to sizes of 1.0 (purple), 0.5 (red), and 0.2 (orange) μm. (B) Ratio of 1.0-µm particles according to carboxylate (purple) and sulfate (blue) surface modification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128364&req=5

fig2: Ratios of various types of particles (z axis) in biofilm-forming strains (x axis) at days 4 and 5 after continuous feeding with particles upon inoculation of the flow cell. All particle numbers (y axis) are standardized to 100 particles of a size of 1.0 µm and carboxylate modification. (A) Ratio of carboxylate particles according to sizes of 1.0 (purple), 0.5 (red), and 0.2 (orange) μm. (B) Ratio of 1.0-µm particles according to carboxylate (purple) and sulfate (blue) surface modification.
Mentions: Particles were incorporated from the medium into the three biofilm-forming strains (Alg+ Pel+ Psl+, Alg+ Pel− Psl+, and Alg+ Pel+ Psl−), with increased uptake of the larger particles relative to the smaller particles. Particles were counted in 4- and 5-day biofilms, and the ratio of 1.0- to 0.5- and 0.2-µm particles was used as a measure of size selectivity in the three biofilm-forming strains (Fig. 2A). The Alg+ Pel+ Psl+ biofilm had 1.0- to 0.5- and 0.2-µm particle ratios of 2.02 ± 0.41 and 4.13 ± 1.24, respectively, while Alg+ Pel− Psl+ biofilm displayed ratios of 1.85 ± 0.55 and 4.81 ± 1.12, respectively. The Alg+ Pel+ Psl− biofilm, which was significantly impaired in biofilm development, had lower 1.0- to 0.5- and 0.2-µm particle ratios of 1.52 ± 0.31 and 2.44 ± 0.73, respectively. It was observed that the preference for 1.0-µm particles over the smaller particles was predominant in the microcolonies (Fig. 3). Hence strains that expressed the Psl polysaccharide and that formed more differentiated biofilms gave higher ratios of 1.0- to 0.5- and 0.2-µm particles.

Bottom Line: Conversely, Pel reduced effective cross-linking within the matrix.The wild-type biofilm decreased in effective cross-linking over time, which would be advantageous for the spreading and colonization of new surfaces.The exopolysaccharides were also found to have profound effects on the spatial organization and integration of P. aeruginosa in a mixed-species biofilm model of P. aeruginosa-Staphylococcus aureus.

View Article: PubMed Central - PubMed

Affiliation: BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, National University of Singapore, Singapore.

Show MeSH
Related in: MedlinePlus