Highly recombinant VGII Cryptococcus gattii population develops clonal outbreak clusters through both sexual macroevolution and asexual microevolution.
Bottom Line: We found that VGIIa/b/c populations show evidence of clonal expansion in the PNW.We also found that the genomes of two basal VGII isolates from HIV(+) patients contain an introgression tract spanning three genes.This work shows that multiple processes can contribute to the emergence of an outbreak.
Affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA email@example.com.Show MeSH
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Mentions: In addition to the shared genomic islands, use of a paired allele compatibility test based on SNP information (AB × ab giving rise to AB, ab, Ab, or αB) provided evidence for sexual reproduction and recombination (Fig. 7A). One hundred random loci were selected using the Genome Analysis Toolkit (GATK) SelectVariants walker out of the 262,614 polymorphic sites in the data set. These loci were collapsed into unique and informative allele combinations, leaving 46 loci, and then compared. This test showed evidence for mating and meiosis occurring between a number of different isolates at the whole-genome level, both with and without the involvement of the three sequenced MATa isolates in this study. In particular, all of the 46 loci tested showed evidence for recombination with at least 13 of the 45 other loci (29%) and as many as 40 out of 45 (89%) (Fig. 7B). Recombination provides strong evidence for the involvement of the sexual cycle in the production of these C. gattii strains, and given the paucity of a isolates in the environment, we hypothesize this involved both a-α bisexual and α-α unisexual reproduction.
Affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA firstname.lastname@example.org.