Limits...
Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression.

Kriegeskorte A, Block D, Drescher M, Windmüller N, Mellmann A, Baum C, Neumann C, Lorè NI, Bragonzi A, Liebau E, Hertel P, Seggewiss J, Becker K, Proctor RA, Peters G, Kahl BC - MBio (2014)

Bottom Line: The expression of genes involved in pyrimidine and purine metabolism and nucleotide interconversion changed significantly.In the era of community-associated methicillin-resistant S. aureus, the therapeutic use of TMP-SMX is increasing.Our study helps to understand the clinical nature of TD-SCVs, which emerge frequently once patients are treated with TMP-SMX.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.

Show MeSH

Related in: MedlinePlus

S. aureus pyrimidine metabolic pathway and the transcriptional activities of respective genes in the ΔthyA mutant. Upregulated genes are labeled in green, while unregulated genes are labeled in black. (I) Pyrimidine ribonucleotide biosynthesis pathway; (II) salvage pathways of nucleotides; (III) external sources of nucleosides. Gene identifier and gene names were reported according to S. aureus COL, accession number NC_002951.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4128360&req=5

fig4: S. aureus pyrimidine metabolic pathway and the transcriptional activities of respective genes in the ΔthyA mutant. Upregulated genes are labeled in green, while unregulated genes are labeled in black. (I) Pyrimidine ribonucleotide biosynthesis pathway; (II) salvage pathways of nucleotides; (III) external sources of nucleosides. Gene identifier and gene names were reported according to S. aureus COL, accession number NC_002951.

Mentions: As de novo thymidylate synthesis is blocked in the ΔthyA mutant, we expected major differences in the pyrimidine/purine metabolic pathways compared to those of the wild-type strain. All genes involved in the anabolic pathway of thymidylate, including carA, carB, pyrB, pyrC, pyrD, pyrE, pyrF pyrG, nrd, ndk, and tmk, were particularly upregulated, which indicates a possible lack of end product repression and an attempt of the cell to compensate thymidylate starvation by increased synthesis of precursors (Table 2, Fig. 4).


Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression.

Kriegeskorte A, Block D, Drescher M, Windmüller N, Mellmann A, Baum C, Neumann C, Lorè NI, Bragonzi A, Liebau E, Hertel P, Seggewiss J, Becker K, Proctor RA, Peters G, Kahl BC - MBio (2014)

S. aureus pyrimidine metabolic pathway and the transcriptional activities of respective genes in the ΔthyA mutant. Upregulated genes are labeled in green, while unregulated genes are labeled in black. (I) Pyrimidine ribonucleotide biosynthesis pathway; (II) salvage pathways of nucleotides; (III) external sources of nucleosides. Gene identifier and gene names were reported according to S. aureus COL, accession number NC_002951.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128360&req=5

fig4: S. aureus pyrimidine metabolic pathway and the transcriptional activities of respective genes in the ΔthyA mutant. Upregulated genes are labeled in green, while unregulated genes are labeled in black. (I) Pyrimidine ribonucleotide biosynthesis pathway; (II) salvage pathways of nucleotides; (III) external sources of nucleosides. Gene identifier and gene names were reported according to S. aureus COL, accession number NC_002951.
Mentions: As de novo thymidylate synthesis is blocked in the ΔthyA mutant, we expected major differences in the pyrimidine/purine metabolic pathways compared to those of the wild-type strain. All genes involved in the anabolic pathway of thymidylate, including carA, carB, pyrB, pyrC, pyrD, pyrE, pyrF pyrG, nrd, ndk, and tmk, were particularly upregulated, which indicates a possible lack of end product repression and an attempt of the cell to compensate thymidylate starvation by increased synthesis of precursors (Table 2, Fig. 4).

Bottom Line: The expression of genes involved in pyrimidine and purine metabolism and nucleotide interconversion changed significantly.In the era of community-associated methicillin-resistant S. aureus, the therapeutic use of TMP-SMX is increasing.Our study helps to understand the clinical nature of TD-SCVs, which emerge frequently once patients are treated with TMP-SMX.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.

Show MeSH
Related in: MedlinePlus