Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression.
Bottom Line: The expression of genes involved in pyrimidine and purine metabolism and nucleotide interconversion changed significantly.In the era of community-associated methicillin-resistant S. aureus, the therapeutic use of TMP-SMX is increasing.Our study helps to understand the clinical nature of TD-SCVs, which emerge frequently once patients are treated with TMP-SMX.
Affiliation: Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.Show MeSH
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Mentions: As de novo thymidylate synthesis is blocked in the ΔthyA mutant, we expected major differences in the pyrimidine/purine metabolic pathways compared to those of the wild-type strain. All genes involved in the anabolic pathway of thymidylate, including carA, carB, pyrB, pyrC, pyrD, pyrE, pyrF pyrG, nrd, ndk, and tmk, were particularly upregulated, which indicates a possible lack of end product repression and an attempt of the cell to compensate thymidylate starvation by increased synthesis of precursors (Table 2, Fig. 4).
Affiliation: Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.