Novel entries in a fungal biofilm matrix encyclopedia.
Bottom Line: Newly described, more abundant polysaccharides included α-1,2 branched α-1,6-mannans (87%) associated with unbranched β-1,6-glucans (13%) in an apparent mannan-glucan complex (MGCx).Importance: This report is the first to decipher the complex and unique macromolecular composition of the Candida biofilm matrix, demonstrate the clinical relevance of matrix components, and show that multiple matrix components are needed for protection from antifungal drugs.The availability of these biochemical analyses provides a unique resource for further functional investigation of the biofilm matrix, a defining trait of this lifestyle.
Affiliation: Department of Medicine, Infectious Diseases, University of Wisconsin, Madison, Wisconsin, USA.Show MeSH
Related in: MedlinePlus
Mentions: Confocal imaging of in vitro biofilms was undertaken to examine the distribution of mannan and β-1,6-glucan matrix components by labeling with monoclonal antibodies developed against and specific for purified matrix components (data not shown). The images demonstrated an extracellular location for each polysaccharide and a distribution throughout the biofilm depth in Fig. 4A to C. The two-channel image color is suggestive of polysaccharide colocalization and consistent with an MGCx (Fig. 4D).
Affiliation: Department of Medicine, Infectious Diseases, University of Wisconsin, Madison, Wisconsin, USA.