Novel entries in a fungal biofilm matrix encyclopedia.
Bottom Line: Newly described, more abundant polysaccharides included α-1,2 branched α-1,6-mannans (87%) associated with unbranched β-1,6-glucans (13%) in an apparent mannan-glucan complex (MGCx).Importance: This report is the first to decipher the complex and unique macromolecular composition of the Candida biofilm matrix, demonstrate the clinical relevance of matrix components, and show that multiple matrix components are needed for protection from antifungal drugs.The availability of these biochemical analyses provides a unique resource for further functional investigation of the biofilm matrix, a defining trait of this lifestyle.
Affiliation: Department of Medicine, Infectious Diseases, University of Wisconsin, Madison, Wisconsin, USA.Show MeSH
Related in: MedlinePlus
Mentions: Monosugar analysis of the total biofilm matrix revealed four relatively abundant monosaccharides, arabinose (Ara), mannose (Man), glucose (Glc), and xylose (Xyl), which accounted for 47.9%, 20%, 12.5%, and 12.6% of the total carbohydrate pool. Initial size exclusion chromatography of the crude matrix yielded two major pools: a high-molecular-weight (HMW) fraction and low-molecular-weight (LMW) fraction that contained 38.3% and 61.7% of the matrix material, respectively (Fig. 2A). Of the total matrix carbohydrates, 27.1% separated in the LMW pool, which contained mostly Ara (52.2%) with smaller amounts of Man, Xyl, and Glc (13.8%, 13.8%, and 12.9%, respectively). The HMW pool contained 21.6% of the total carbohydrate and consisted primarily of Man (89.2%) and Glc (8.6%).
Affiliation: Department of Medicine, Infectious Diseases, University of Wisconsin, Madison, Wisconsin, USA.