Antigen export during liver infection of the malaria parasite augments protective immunity.
Bottom Line: A fundamental question is whether recognition by effector CD8(+) T cells is restricted to sporozoite surface antigens or extends to parasite proteins that are synthesized during the extensive parasite expansion phase in the liver.Using a surrogate model antigen, we found that a cytoplasmic antigen is able to induce robust protective CD8(+) T-cell responses, but protein export further enhances immunogenicity and protection.Our results show that a cytoplasmic localization does not exclude a protein's candidacy for malaria subunit vaccines and that protein secretion can enhance protective immunity.
Affiliation: Parasitology Unit, Max Planck Institute for Infection Biology, Berlin, Germany firstname.lastname@example.org.Show MeSH
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Mentions: To address the functional relevance of antigen-specific CD8+ and CD4+ T cells to protection in this immunization and infection model, we tested the contribution of OVA-specific T cells to inhibition of Plasmodium liver-stage development in vivo. We transferred 2 × 105 nonactivated OT-I (CD8) and OT-II (CD4) T cells each into mice, and 24 h later, immunized the recipient animals with 10,000 irradiated normal or transgenic OVA sporozoites (Fig. 5). Ten days later, the mice were challenged with the respective sporozoite lines used for immunization. Control mice did not receive OVA-specific T cells before immunization.
Affiliation: Parasitology Unit, Max Planck Institute for Infection Biology, Berlin, Germany email@example.com.