Antivirulence activity of the human gut metabolome.
Bottom Line: Importance: Microbes can communicate through the production and sensing of small molecules.Within the complex ecosystem formed by commensal microbes living in and on the human body, it is likely that these molecular messages are used extensively during the interactions between different microbial species as well as with host cells.Deciphering such a molecular dialect will be fundamental to our understanding of host-microbe interactions in health and disease and may prove useful for the design of new therapeutic strategies that target these mechanisms of communication.
Affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.Show MeSH
Related in: MedlinePlus
Mentions: Collectively, our results showed that the human gut metabolome contains a molecule (or molecules) that acts as a strong inhibitor of Salmonella host cell invasion. In order to determine if this is specific to the human gut metabolome or a conserved feature among mammals, we tested the effect of molecules from the murine gut metabolome on Salmonella invasion gene expression during late logarithmic growth. Fresh feces of 129S1/SvImJ Nramp1−/− and Swiss Webster mice were extracted essentially as described for human samples and tested for effects on hilA expression. The results showed that the phenotype of hilA repression elicited by the gut metabolome is not exclusive to humans; fecal extracts from murine feces were also strong repressors of hilA (Fig. 5).
Affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.