Nonhomologous recombination between defective poliovirus and coxsackievirus genomes suggests a new model of genetic plasticity for picornaviruses.
Bottom Line: We found some homologous (H) recombinants and, mostly, nonhomologous (NH) recombinants presenting duplications of parental sequences preferentially located in the regions encoding proteins 2A, 2B, and 3A.For further studies of the genetic exchanges between PV and CA17, we have developed a model of recombination, making it possible to rescue defective PV RNA genomes with a short deletion by cotransfecting cells with the defective PV genome and CA17 genomic RNA.Numerous recombinants were found, including homologous PV/CA17 recombinants, but mostly nonhomologous recombinants presenting duplications of parental sequences preferentially located in particular regions.
Affiliation: Institut Pasteur, Biologie des Virus Entériques, Paris, France INSERM U994, Institut National de La Santé et de La Recherche Médicale, Paris, France.Show MeSH
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Mentions: Surprisingly, only 33% of S2CA17 and 30% of S2CA17Δ recombinants were the products of H recombination events, the others resulting from NH recombination events. An analysis of the recombination sites showed them to be located in the region encoding the C-terminal part of VP1 and the nonstructural protein P2 and P3 coding regions (Fig. 3A; see also Tables S1 and S2 in the supplemental material). Examples for H and NH recombination sites are shown in Fig. S1A and B, respectively. The proportions of H and NH recombinants and the locations of recombination sites were similar for S2CA17 and S2CA17Δ. We therefore present the data for S2CA17 and S2CA17Δ together.
Affiliation: Institut Pasteur, Biologie des Virus Entériques, Paris, France INSERM U994, Institut National de La Santé et de La Recherche Médicale, Paris, France.