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Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients.

Della Vittoria Scarpati G, Calura E, Di Marino M, Romualdi C, Beltrame L, Malapelle U, Troncone G, De Stefano A, Pepe S, De Placido S, D'Incalci M, Marchini S, Carlomagno C - Biomed Res Int (2014)

Bottom Line: Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p.No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.

ABSTRACT
Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

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RT-PCR validation of DE miRNA in tumor versus stroma matched samples. Box plots show the median expression levels of the 10 miRNAs confirmed as upregulated in tumor samples, estimated in terms of normalized fluorescence intensity. Significant differences at Wilcoxon Rank test with a percentile range of 2.5–97.5 are reported (****P value < 0.0001).
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fig3: RT-PCR validation of DE miRNA in tumor versus stroma matched samples. Box plots show the median expression levels of the 10 miRNAs confirmed as upregulated in tumor samples, estimated in terms of normalized fluorescence intensity. Significant differences at Wilcoxon Rank test with a percentile range of 2.5–97.5 are reported (****P value < 0.0001).

Mentions: To assess the reproducibility and robustness of the miRNA signature identified by array analysis, we measured by qRT-PCR the expression of 13 out of 26 top ranked miRNAs differentially expressed in the tumor and the stroma (Supplementary Table 3). The Wilcoxon rank test confirmed the difference in expression level for 10 of the 13 selected miRNAs. Box plots reported in Figure 3 show the median distribution levels of normalized fluorescence intensity and the IQ-R of the 10 upregulated or downregulated miRNAs obtained by qRT-PCR analysis. Of note, 9 out of the 10 miRNAs analyzed are more expressed in the tumor than in the stroma, whereas 1 is downregulated.


Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients.

Della Vittoria Scarpati G, Calura E, Di Marino M, Romualdi C, Beltrame L, Malapelle U, Troncone G, De Stefano A, Pepe S, De Placido S, D'Incalci M, Marchini S, Carlomagno C - Biomed Res Int (2014)

RT-PCR validation of DE miRNA in tumor versus stroma matched samples. Box plots show the median expression levels of the 10 miRNAs confirmed as upregulated in tumor samples, estimated in terms of normalized fluorescence intensity. Significant differences at Wilcoxon Rank test with a percentile range of 2.5–97.5 are reported (****P value < 0.0001).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128171&req=5

fig3: RT-PCR validation of DE miRNA in tumor versus stroma matched samples. Box plots show the median expression levels of the 10 miRNAs confirmed as upregulated in tumor samples, estimated in terms of normalized fluorescence intensity. Significant differences at Wilcoxon Rank test with a percentile range of 2.5–97.5 are reported (****P value < 0.0001).
Mentions: To assess the reproducibility and robustness of the miRNA signature identified by array analysis, we measured by qRT-PCR the expression of 13 out of 26 top ranked miRNAs differentially expressed in the tumor and the stroma (Supplementary Table 3). The Wilcoxon rank test confirmed the difference in expression level for 10 of the 13 selected miRNAs. Box plots reported in Figure 3 show the median distribution levels of normalized fluorescence intensity and the IQ-R of the 10 upregulated or downregulated miRNAs obtained by qRT-PCR analysis. Of note, 9 out of the 10 miRNAs analyzed are more expressed in the tumor than in the stroma, whereas 1 is downregulated.

Bottom Line: Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p.No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.

ABSTRACT
Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

Show MeSH
Related in: MedlinePlus