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Effects of two bioactive materials on survival and osteoblastic differentiation of human mesenchymal stem cells.

Hengameh A, Reyhaneh D, Nima MM, Hamed H - J Conserv Dent (2014)

Bottom Line: P < 0.05 was considered as statistically significant.Cell viability was not significantly different.Osteocalcin gene expression was significantly higher in the CEM group compared to the control (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Dental Research Center, Institute of Dental Sciences, Dental School, Shahid Beheshti University of Medical Science, Tehran, Iran.

ABSTRACT

Objectives: Activation of mineralization process in periradicular tissues following the injuries, is important in repair mechanisms. The objective of this study was to investigate the effects of CEM cement on survival and mineralization of human mesenchymal stem cells (hMSCs) and compare it with MTA.

Materials and methods: hMSCs that were planted on test material extracts and culture media were the experimental and control groups, respectively. The cytotoxicity of these materials was investigated using Methyl thiazol tetrazolium assay. After 7 days, alizarin red staining, alkaline phosphatase (ALP) assays, and qRT-PCR were used to assess the mineralization, expression of ALP, and gene expression (collagen type 1 and osteocalcin), respectively. The results were evaluated by ANOVA analysis and multiple comparisons test. P < 0.05 was considered as statistically significant.

Results: Cell viability was not significantly different. Alizarin red and alkaline phosphatase staining showed mineralization in all three groups. In qRT-PCR, the expression of collagen type 1 is not significantly different among the three groups. Osteocalcin gene expression was significantly higher in the CEM group compared to the control (P < 0.05).

Conclusion: CEM cement has acceptable toxicity and could induce mineralization process and enhance osteocalcin gene expression which is associated with mineralization in hMSCs.

No MeSH data available.


Related in: MedlinePlus

Micrographs of hMSCs after 7 days. (A-C: Alizarin red staining of specimens and D-F: Expression of ALP in the samples) Mineralization nodule formations were observed in the group of MTA (a), CEM cement (b), and Control (c). Furthermore, expression of ALP was detected in all three groups (MTA (d), CEM cement (e), and control (f))
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Figure 1: Micrographs of hMSCs after 7 days. (A-C: Alizarin red staining of specimens and D-F: Expression of ALP in the samples) Mineralization nodule formations were observed in the group of MTA (a), CEM cement (b), and Control (c). Furthermore, expression of ALP was detected in all three groups (MTA (d), CEM cement (e), and control (f))

Mentions: Alizarin red staining nodules were observed in all the groups (MTA, CEM cement, and control), mineralization nodule formation in the CEM cement group was the most prominent [Figure 1].


Effects of two bioactive materials on survival and osteoblastic differentiation of human mesenchymal stem cells.

Hengameh A, Reyhaneh D, Nima MM, Hamed H - J Conserv Dent (2014)

Micrographs of hMSCs after 7 days. (A-C: Alizarin red staining of specimens and D-F: Expression of ALP in the samples) Mineralization nodule formations were observed in the group of MTA (a), CEM cement (b), and Control (c). Furthermore, expression of ALP was detected in all three groups (MTA (d), CEM cement (e), and control (f))
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4127694&req=5

Figure 1: Micrographs of hMSCs after 7 days. (A-C: Alizarin red staining of specimens and D-F: Expression of ALP in the samples) Mineralization nodule formations were observed in the group of MTA (a), CEM cement (b), and Control (c). Furthermore, expression of ALP was detected in all three groups (MTA (d), CEM cement (e), and control (f))
Mentions: Alizarin red staining nodules were observed in all the groups (MTA, CEM cement, and control), mineralization nodule formation in the CEM cement group was the most prominent [Figure 1].

Bottom Line: P < 0.05 was considered as statistically significant.Cell viability was not significantly different.Osteocalcin gene expression was significantly higher in the CEM group compared to the control (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Dental Research Center, Institute of Dental Sciences, Dental School, Shahid Beheshti University of Medical Science, Tehran, Iran.

ABSTRACT

Objectives: Activation of mineralization process in periradicular tissues following the injuries, is important in repair mechanisms. The objective of this study was to investigate the effects of CEM cement on survival and mineralization of human mesenchymal stem cells (hMSCs) and compare it with MTA.

Materials and methods: hMSCs that were planted on test material extracts and culture media were the experimental and control groups, respectively. The cytotoxicity of these materials was investigated using Methyl thiazol tetrazolium assay. After 7 days, alizarin red staining, alkaline phosphatase (ALP) assays, and qRT-PCR were used to assess the mineralization, expression of ALP, and gene expression (collagen type 1 and osteocalcin), respectively. The results were evaluated by ANOVA analysis and multiple comparisons test. P < 0.05 was considered as statistically significant.

Results: Cell viability was not significantly different. Alizarin red and alkaline phosphatase staining showed mineralization in all three groups. In qRT-PCR, the expression of collagen type 1 is not significantly different among the three groups. Osteocalcin gene expression was significantly higher in the CEM group compared to the control (P < 0.05).

Conclusion: CEM cement has acceptable toxicity and could induce mineralization process and enhance osteocalcin gene expression which is associated with mineralization in hMSCs.

No MeSH data available.


Related in: MedlinePlus