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Abarema cochliacarpos extract decreases the inflammatory process and skeletal muscle injury induced by Bothrops leucurus venom.

Saturnino-Oliveira J, Santos Ddo C, Guimarães AG, Santos Dias A, Tomaz MA, Monteiro-Machado M, Estevam CS, De Lucca Júnior W, Maria DA, Melo PA, Araújo AA, Santos MR, Almeida JR, Oliveira Rde C, Pereira de Oliveira A, Quintans Júnior LJ - Biomed Res Int (2014)

Bottom Line: However, treatment with antivenom has limited effectiveness against venoms' local effects.Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom.Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil.

ABSTRACT
Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

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In vivo myotoxicity. The figure shows the effect of EAc and dexamethasone on B. leucurus myotoxic activity in vivo. Results show plasma CK activity 2 hours after injection of the venom alone or followed by treatment with i.v. dexamethasone or EAc by oral gavage. Data report means ± SEM (N = 5). *P < 0.05 for the difference between treated groups and the venom (Student's t-test).
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fig4: In vivo myotoxicity. The figure shows the effect of EAc and dexamethasone on B. leucurus myotoxic activity in vivo. Results show plasma CK activity 2 hours after injection of the venom alone or followed by treatment with i.v. dexamethasone or EAc by oral gavage. Data report means ± SEM (N = 5). *P < 0.05 for the difference between treated groups and the venom (Student's t-test).

Mentions: Mice injected intramuscularly with the venom of B. leucurus (1 mg/kg) presented, 2 hours after venom injection, an increased activity of CK in plasma, which ranged from 127.75 ± 1.59 U/L (n = 5) in the group receiving the PSS solution up to 877.70 ± 21.54 U/L (n = 5) in the group receiving the venom. Oral treatment of mice with the different doses of EAc partially inhibited the in vivo myotoxic activity of the venom (Figure 4).


Abarema cochliacarpos extract decreases the inflammatory process and skeletal muscle injury induced by Bothrops leucurus venom.

Saturnino-Oliveira J, Santos Ddo C, Guimarães AG, Santos Dias A, Tomaz MA, Monteiro-Machado M, Estevam CS, De Lucca Júnior W, Maria DA, Melo PA, Araújo AA, Santos MR, Almeida JR, Oliveira Rde C, Pereira de Oliveira A, Quintans Júnior LJ - Biomed Res Int (2014)

In vivo myotoxicity. The figure shows the effect of EAc and dexamethasone on B. leucurus myotoxic activity in vivo. Results show plasma CK activity 2 hours after injection of the venom alone or followed by treatment with i.v. dexamethasone or EAc by oral gavage. Data report means ± SEM (N = 5). *P < 0.05 for the difference between treated groups and the venom (Student's t-test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4127289&req=5

fig4: In vivo myotoxicity. The figure shows the effect of EAc and dexamethasone on B. leucurus myotoxic activity in vivo. Results show plasma CK activity 2 hours after injection of the venom alone or followed by treatment with i.v. dexamethasone or EAc by oral gavage. Data report means ± SEM (N = 5). *P < 0.05 for the difference between treated groups and the venom (Student's t-test).
Mentions: Mice injected intramuscularly with the venom of B. leucurus (1 mg/kg) presented, 2 hours after venom injection, an increased activity of CK in plasma, which ranged from 127.75 ± 1.59 U/L (n = 5) in the group receiving the PSS solution up to 877.70 ± 21.54 U/L (n = 5) in the group receiving the venom. Oral treatment of mice with the different doses of EAc partially inhibited the in vivo myotoxic activity of the venom (Figure 4).

Bottom Line: However, treatment with antivenom has limited effectiveness against venoms' local effects.Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom.Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil.

ABSTRACT
Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

Show MeSH
Related in: MedlinePlus