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Abarema cochliacarpos extract decreases the inflammatory process and skeletal muscle injury induced by Bothrops leucurus venom.

Saturnino-Oliveira J, Santos Ddo C, Guimarães AG, Santos Dias A, Tomaz MA, Monteiro-Machado M, Estevam CS, De Lucca Júnior W, Maria DA, Melo PA, Araújo AA, Santos MR, Almeida JR, Oliveira Rde C, Pereira de Oliveira A, Quintans Júnior LJ - Biomed Res Int (2014)

Bottom Line: However, treatment with antivenom has limited effectiveness against venoms' local effects.Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom.Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil.

ABSTRACT
Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

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Related in: MedlinePlus

Functional activity. Time spent by mice on the rotarod (8 rpm) before and after receiving B. leucurus venom (1.0 mg/kg). The animals were treated with oral EAc at 100 mg/kg, 200 mg/kg, and 400 mg/kg and i.v. dexamethasone (2 mg/kg) 5 min after venom injection. Time zero represents data obtained before venom injection. Data report means ± SEM (n = 6). *P < 0.05 versus venom group (Student's t-test).
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fig3: Functional activity. Time spent by mice on the rotarod (8 rpm) before and after receiving B. leucurus venom (1.0 mg/kg). The animals were treated with oral EAc at 100 mg/kg, 200 mg/kg, and 400 mg/kg and i.v. dexamethasone (2 mg/kg) 5 min after venom injection. Time zero represents data obtained before venom injection. Data report means ± SEM (n = 6). *P < 0.05 versus venom group (Student's t-test).

Mentions: After B. leucurus venom injection, all animals, including those receiving treatment, showed a decrease in functional ability to stand on the rotarod. However, on the first and third days after injection, mice receiving venom only or venom treated with 100 mg/kg and 200 mg/kg EAc showed a more pronounced decrease, compared to animals treated with Dexa or 400 mg/kg EAc. This result shows that EAc decreased the impact of venom on motor functional activity. By day 7, all animals, including those that received only venom injection without any treatment, were able to stand on the rotarod as long as the control mice, showing recovered muscle function (Figure 3).


Abarema cochliacarpos extract decreases the inflammatory process and skeletal muscle injury induced by Bothrops leucurus venom.

Saturnino-Oliveira J, Santos Ddo C, Guimarães AG, Santos Dias A, Tomaz MA, Monteiro-Machado M, Estevam CS, De Lucca Júnior W, Maria DA, Melo PA, Araújo AA, Santos MR, Almeida JR, Oliveira Rde C, Pereira de Oliveira A, Quintans Júnior LJ - Biomed Res Int (2014)

Functional activity. Time spent by mice on the rotarod (8 rpm) before and after receiving B. leucurus venom (1.0 mg/kg). The animals were treated with oral EAc at 100 mg/kg, 200 mg/kg, and 400 mg/kg and i.v. dexamethasone (2 mg/kg) 5 min after venom injection. Time zero represents data obtained before venom injection. Data report means ± SEM (n = 6). *P < 0.05 versus venom group (Student's t-test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4127289&req=5

fig3: Functional activity. Time spent by mice on the rotarod (8 rpm) before and after receiving B. leucurus venom (1.0 mg/kg). The animals were treated with oral EAc at 100 mg/kg, 200 mg/kg, and 400 mg/kg and i.v. dexamethasone (2 mg/kg) 5 min after venom injection. Time zero represents data obtained before venom injection. Data report means ± SEM (n = 6). *P < 0.05 versus venom group (Student's t-test).
Mentions: After B. leucurus venom injection, all animals, including those receiving treatment, showed a decrease in functional ability to stand on the rotarod. However, on the first and third days after injection, mice receiving venom only or venom treated with 100 mg/kg and 200 mg/kg EAc showed a more pronounced decrease, compared to animals treated with Dexa or 400 mg/kg EAc. This result shows that EAc decreased the impact of venom on motor functional activity. By day 7, all animals, including those that received only venom injection without any treatment, were able to stand on the rotarod as long as the control mice, showing recovered muscle function (Figure 3).

Bottom Line: However, treatment with antivenom has limited effectiveness against venoms' local effects.Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom.Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil.

ABSTRACT
Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

Show MeSH
Related in: MedlinePlus