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Effects of pristane alone or combined with chloroquine on macrophage activation, oxidative stress, and TH1/TH2 skewness.

Ouyang Q, Huang Z, Wang Z, Chen X, Ni J, Lin L - J Immunol Res (2014)

Bottom Line: And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Second Affiliated Hospital and Second Clinical Medical College, Fujian Medical University, Zhongshan North Road 34, Quanzhou, Fujian 362000, China.

ABSTRACT
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

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Effects of chloroquine on oxidative stress induced by pristane. Pristane increased the content of superoxide (red, DHE stain) and MDA and decreased the levels of SOD, GSH, and CAT, which could be ameliorated by coadministration of pristane and CQ. Original magnification ×400. CQ: chloroquine; MDA: malondialdehyde; SOD: superoxide dismutase; GSH: glutathione; CAT: catalase. *P < 0.05 versus the corresponding control mice, #P < 0.05 versus mice exposure to pristane for 12 weeks.
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fig4: Effects of chloroquine on oxidative stress induced by pristane. Pristane increased the content of superoxide (red, DHE stain) and MDA and decreased the levels of SOD, GSH, and CAT, which could be ameliorated by coadministration of pristane and CQ. Original magnification ×400. CQ: chloroquine; MDA: malondialdehyde; SOD: superoxide dismutase; GSH: glutathione; CAT: catalase. *P < 0.05 versus the corresponding control mice, #P < 0.05 versus mice exposure to pristane for 12 weeks.

Mentions: During phagocytosis, macrophages produce reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide. To investigate the effects of pristane on oxidative stress, the levels of ROS, MDA, and endogenous antioxidant enzymes (SOD, GSH, and CAT) in serum were analyzed (Figure 4). Mice exposure to pristane for 12 or 24 weeks illustrated dramatically increased superoxide anion as manifested by dihydroethidium fluorescence staining. Meanwhile, the levels of MDA were significantly increased by 1.11-fold and 0.60-fold compared with that in the normal mice. CQ treatment for 12 weeks significantly reduced the MDA concentration. Alternatively, the activity of SOD, GSH, and CAT was reduced following pristane injection and this reduction was attenuated by CQ treatment.


Effects of pristane alone or combined with chloroquine on macrophage activation, oxidative stress, and TH1/TH2 skewness.

Ouyang Q, Huang Z, Wang Z, Chen X, Ni J, Lin L - J Immunol Res (2014)

Effects of chloroquine on oxidative stress induced by pristane. Pristane increased the content of superoxide (red, DHE stain) and MDA and decreased the levels of SOD, GSH, and CAT, which could be ameliorated by coadministration of pristane and CQ. Original magnification ×400. CQ: chloroquine; MDA: malondialdehyde; SOD: superoxide dismutase; GSH: glutathione; CAT: catalase. *P < 0.05 versus the corresponding control mice, #P < 0.05 versus mice exposure to pristane for 12 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127244&req=5

fig4: Effects of chloroquine on oxidative stress induced by pristane. Pristane increased the content of superoxide (red, DHE stain) and MDA and decreased the levels of SOD, GSH, and CAT, which could be ameliorated by coadministration of pristane and CQ. Original magnification ×400. CQ: chloroquine; MDA: malondialdehyde; SOD: superoxide dismutase; GSH: glutathione; CAT: catalase. *P < 0.05 versus the corresponding control mice, #P < 0.05 versus mice exposure to pristane for 12 weeks.
Mentions: During phagocytosis, macrophages produce reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide. To investigate the effects of pristane on oxidative stress, the levels of ROS, MDA, and endogenous antioxidant enzymes (SOD, GSH, and CAT) in serum were analyzed (Figure 4). Mice exposure to pristane for 12 or 24 weeks illustrated dramatically increased superoxide anion as manifested by dihydroethidium fluorescence staining. Meanwhile, the levels of MDA were significantly increased by 1.11-fold and 0.60-fold compared with that in the normal mice. CQ treatment for 12 weeks significantly reduced the MDA concentration. Alternatively, the activity of SOD, GSH, and CAT was reduced following pristane injection and this reduction was attenuated by CQ treatment.

Bottom Line: And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Second Affiliated Hospital and Second Clinical Medical College, Fujian Medical University, Zhongshan North Road 34, Quanzhou, Fujian 362000, China.

ABSTRACT
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

Show MeSH
Related in: MedlinePlus