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Effects of pristane alone or combined with chloroquine on macrophage activation, oxidative stress, and TH1/TH2 skewness.

Ouyang Q, Huang Z, Wang Z, Chen X, Ni J, Lin L - J Immunol Res (2014)

Bottom Line: And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Second Affiliated Hospital and Second Clinical Medical College, Fujian Medical University, Zhongshan North Road 34, Quanzhou, Fujian 362000, China.

ABSTRACT
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

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Macrophage phagocytic function revealed by oil red O staining. Peritoneal macrophages were exposed to 500 ng/mL ox-LDL for 24 h.
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fig3: Macrophage phagocytic function revealed by oil red O staining. Peritoneal macrophages were exposed to 500 ng/mL ox-LDL for 24 h.

Mentions: To further confirm the effects of pristane on the macrophage phagocytic function, peritoneal macrophages were collected to detect their lipid phagocytic activity (Figure 3). As compared to control mice, the phagocytic index was significantly higher after exposure to pristane for 12 weeks (95.2 ± 7.2% versus 35.4 ± 5.1%, P < 0.01) or for 24 weeks (78.6 ± 8.4% versus 31.7 ± 4.9%, P < 0.01). Phagocytosis was inhibited in peritoneal macrophages of chloroquine treated mice (65.9 ± 9.3%).


Effects of pristane alone or combined with chloroquine on macrophage activation, oxidative stress, and TH1/TH2 skewness.

Ouyang Q, Huang Z, Wang Z, Chen X, Ni J, Lin L - J Immunol Res (2014)

Macrophage phagocytic function revealed by oil red O staining. Peritoneal macrophages were exposed to 500 ng/mL ox-LDL for 24 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127244&req=5

fig3: Macrophage phagocytic function revealed by oil red O staining. Peritoneal macrophages were exposed to 500 ng/mL ox-LDL for 24 h.
Mentions: To further confirm the effects of pristane on the macrophage phagocytic function, peritoneal macrophages were collected to detect their lipid phagocytic activity (Figure 3). As compared to control mice, the phagocytic index was significantly higher after exposure to pristane for 12 weeks (95.2 ± 7.2% versus 35.4 ± 5.1%, P < 0.01) or for 24 weeks (78.6 ± 8.4% versus 31.7 ± 4.9%, P < 0.01). Phagocytosis was inhibited in peritoneal macrophages of chloroquine treated mice (65.9 ± 9.3%).

Bottom Line: And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Second Affiliated Hospital and Second Clinical Medical College, Fujian Medical University, Zhongshan North Road 34, Quanzhou, Fujian 362000, China.

ABSTRACT
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

Show MeSH
Related in: MedlinePlus