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Effects of pristane alone or combined with chloroquine on macrophage activation, oxidative stress, and TH1/TH2 skewness.

Ouyang Q, Huang Z, Wang Z, Chen X, Ni J, Lin L - J Immunol Res (2014)

Bottom Line: And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Second Affiliated Hospital and Second Clinical Medical College, Fujian Medical University, Zhongshan North Road 34, Quanzhou, Fujian 362000, China.

ABSTRACT
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

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Effects of pristane alone or combined with chloroquine on cytological characteristics in blood and bone marrow. Wright-Giemsa staining was performed in peripheral blood (a) and bone marrow (b) smears. Hemophagocytosis (arrows), poikilocytosis, polychromasia, hypochromia, and anisocytosis of erythrocytes were observed in blood smear of pristane-treated mice. Bone marrow cytology depicted hypocellularity in erythroid progenitors, poikilocytosis, and nonerythroid lineage clonal expansion following treatment with pristane for 12 weeks or 24 weeks. And the severity of poikilocytosis was attenuated in the presence of chloroquine.
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fig2: Effects of pristane alone or combined with chloroquine on cytological characteristics in blood and bone marrow. Wright-Giemsa staining was performed in peripheral blood (a) and bone marrow (b) smears. Hemophagocytosis (arrows), poikilocytosis, polychromasia, hypochromia, and anisocytosis of erythrocytes were observed in blood smear of pristane-treated mice. Bone marrow cytology depicted hypocellularity in erythroid progenitors, poikilocytosis, and nonerythroid lineage clonal expansion following treatment with pristane for 12 weeks or 24 weeks. And the severity of poikilocytosis was attenuated in the presence of chloroquine.

Mentions: For morphological analysis, abnormal erythrocyte morphology such as stomatocytes, acanthocytes, schistocytes, and dacryocytes could be observed following pristane injection. And the severity of poikilocytosis was attenuated in the presence of chloroquine (Figure 2). Hemophagocytosis was also detected on peripheral blood smear. Concurrently, the bone marrow cells displayed clonal expansion in non-erythroid lineage progenitors and hypoplasia in erythroid progenitors. The myeloid/erythroid (M/E) ratio was significantly elevated following exposure to pristane for 12 weeks (4.53 ± 0.92 versus 2.12 ± 0.45) or 24 weeks (3.71 ± 0.78 versus 2.33 ± 0.51), as compared to their corresponding controls. The severity of poikilocytosis and the ratio of M/E were attenuated in the presence of chloroquine. Contrastingly, peripheral blood and bone marrow smears from control mice showed normal RBC morphology.


Effects of pristane alone or combined with chloroquine on macrophage activation, oxidative stress, and TH1/TH2 skewness.

Ouyang Q, Huang Z, Wang Z, Chen X, Ni J, Lin L - J Immunol Res (2014)

Effects of pristane alone or combined with chloroquine on cytological characteristics in blood and bone marrow. Wright-Giemsa staining was performed in peripheral blood (a) and bone marrow (b) smears. Hemophagocytosis (arrows), poikilocytosis, polychromasia, hypochromia, and anisocytosis of erythrocytes were observed in blood smear of pristane-treated mice. Bone marrow cytology depicted hypocellularity in erythroid progenitors, poikilocytosis, and nonerythroid lineage clonal expansion following treatment with pristane for 12 weeks or 24 weeks. And the severity of poikilocytosis was attenuated in the presence of chloroquine.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127244&req=5

fig2: Effects of pristane alone or combined with chloroquine on cytological characteristics in blood and bone marrow. Wright-Giemsa staining was performed in peripheral blood (a) and bone marrow (b) smears. Hemophagocytosis (arrows), poikilocytosis, polychromasia, hypochromia, and anisocytosis of erythrocytes were observed in blood smear of pristane-treated mice. Bone marrow cytology depicted hypocellularity in erythroid progenitors, poikilocytosis, and nonerythroid lineage clonal expansion following treatment with pristane for 12 weeks or 24 weeks. And the severity of poikilocytosis was attenuated in the presence of chloroquine.
Mentions: For morphological analysis, abnormal erythrocyte morphology such as stomatocytes, acanthocytes, schistocytes, and dacryocytes could be observed following pristane injection. And the severity of poikilocytosis was attenuated in the presence of chloroquine (Figure 2). Hemophagocytosis was also detected on peripheral blood smear. Concurrently, the bone marrow cells displayed clonal expansion in non-erythroid lineage progenitors and hypoplasia in erythroid progenitors. The myeloid/erythroid (M/E) ratio was significantly elevated following exposure to pristane for 12 weeks (4.53 ± 0.92 versus 2.12 ± 0.45) or 24 weeks (3.71 ± 0.78 versus 2.33 ± 0.51), as compared to their corresponding controls. The severity of poikilocytosis and the ratio of M/E were attenuated in the presence of chloroquine. Contrastingly, peripheral blood and bone marrow smears from control mice showed normal RBC morphology.

Bottom Line: And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Department, Second Affiliated Hospital and Second Clinical Medical College, Fujian Medical University, Zhongshan North Road 34, Quanzhou, Fujian 362000, China.

ABSTRACT
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice. Those mice were treated with pristane alone or combined with chloroquine. Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined. And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed. However, chloroquine supplementation showed a remarkable amelioration of these abnormalities. Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.

Show MeSH
Related in: MedlinePlus