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Chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine.

Koriem KM, Soliman RE - J Toxicol (2014)

Bottom Line: Thirty-two male albino rats were divided into 4 equal groups.Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period.Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Medical Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12622, Egypt ; Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), No. 6 Tingkat 1, Persiaran Seksyen 4/9, Bandar Putra Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.

ABSTRACT
Methamphetamine intoxication can cause acute hepatic failure. Chlorogenic and caftaric acids are the major dietary polyphenols present in various foods. The aim of this study was to evaluate the protective role of chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine in rats. Thirty-two male albino rats were divided into 4 equal groups. Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period. Groups 2, 3, and 4 were injected (i.p) with methamphetamine (10 mg/kg) twice a day over seven-day period, where groups 3 and 4 were injected (i.p) with 60 mg/kg chlorogenic acid and 40 mg/kg caftaric acid, respectively, one day before methamphetamine injections. Methamphetamine increased serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, low-density lipoprotein, and triglycerides. Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels. Chlorogenic and caftaric acids prior to methamphetamine injections restored all the above parameters to normal values. In conclusion, chlorogenic and caftaric acids before methamphetamine injections prevented liver toxicity and oxidative stress where chlorogenic acid was more effective.

No MeSH data available.


Related in: MedlinePlus

A photomicrograph of section of liver showing the following. (a) Control: the glycogen particles appear accumulated (arrowhead) in the cytoplasm. (b) Rats injected with METH showing the polysaccharides inclusions that displayed diffuse stain ability (arrowhead). A few number of the hepatocytes display dense stain ability compared to the others. (c) Rats injected with METH in combination with chlorogenic acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300). (d) Rats injected with METH in combination with caftaric acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300).
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fig2: A photomicrograph of section of liver showing the following. (a) Control: the glycogen particles appear accumulated (arrowhead) in the cytoplasm. (b) Rats injected with METH showing the polysaccharides inclusions that displayed diffuse stain ability (arrowhead). A few number of the hepatocytes display dense stain ability compared to the others. (c) Rats injected with METH in combination with chlorogenic acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300). (d) Rats injected with METH in combination with caftaric acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300).

Mentions: Examination of liver of control rats stained with Periodic Acid Schiff's (PAS) technique showed the abundance of polysaccharide materials in the hepatocytes. The polysaccharides particles appear accumulated at one side of the cytoplasm, leaving the other side. In the liver lobule, the hepatocytes at the periphery appear markedly rich in glycogen particles if compared with the pericentral cells (Figure 2(a)). Successive daily METH injections induced diffuse stain ability of the positive PAS materials of the hepatocytes of the METH-treated rats. A few of the hepatocytes displayed denser stain ability than the others (Figure 2(b)). In rats' pretreated with chlorogenic or caftaric acid before METH-injections, the positive PAS materials of the hepatocytes appeared more or less as normal (Figures 2(c) and 2(d)).


Chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine.

Koriem KM, Soliman RE - J Toxicol (2014)

A photomicrograph of section of liver showing the following. (a) Control: the glycogen particles appear accumulated (arrowhead) in the cytoplasm. (b) Rats injected with METH showing the polysaccharides inclusions that displayed diffuse stain ability (arrowhead). A few number of the hepatocytes display dense stain ability compared to the others. (c) Rats injected with METH in combination with chlorogenic acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300). (d) Rats injected with METH in combination with caftaric acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127234&req=5

fig2: A photomicrograph of section of liver showing the following. (a) Control: the glycogen particles appear accumulated (arrowhead) in the cytoplasm. (b) Rats injected with METH showing the polysaccharides inclusions that displayed diffuse stain ability (arrowhead). A few number of the hepatocytes display dense stain ability compared to the others. (c) Rats injected with METH in combination with chlorogenic acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300). (d) Rats injected with METH in combination with caftaric acid showing the polysaccharides inclusions (arrowhead) that appear more or less as control (PAS ×300).
Mentions: Examination of liver of control rats stained with Periodic Acid Schiff's (PAS) technique showed the abundance of polysaccharide materials in the hepatocytes. The polysaccharides particles appear accumulated at one side of the cytoplasm, leaving the other side. In the liver lobule, the hepatocytes at the periphery appear markedly rich in glycogen particles if compared with the pericentral cells (Figure 2(a)). Successive daily METH injections induced diffuse stain ability of the positive PAS materials of the hepatocytes of the METH-treated rats. A few of the hepatocytes displayed denser stain ability than the others (Figure 2(b)). In rats' pretreated with chlorogenic or caftaric acid before METH-injections, the positive PAS materials of the hepatocytes appeared more or less as normal (Figures 2(c) and 2(d)).

Bottom Line: Thirty-two male albino rats were divided into 4 equal groups.Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period.Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Medical Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12622, Egypt ; Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), No. 6 Tingkat 1, Persiaran Seksyen 4/9, Bandar Putra Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.

ABSTRACT
Methamphetamine intoxication can cause acute hepatic failure. Chlorogenic and caftaric acids are the major dietary polyphenols present in various foods. The aim of this study was to evaluate the protective role of chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine in rats. Thirty-two male albino rats were divided into 4 equal groups. Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period. Groups 2, 3, and 4 were injected (i.p) with methamphetamine (10 mg/kg) twice a day over seven-day period, where groups 3 and 4 were injected (i.p) with 60 mg/kg chlorogenic acid and 40 mg/kg caftaric acid, respectively, one day before methamphetamine injections. Methamphetamine increased serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, low-density lipoprotein, and triglycerides. Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels. Chlorogenic and caftaric acids prior to methamphetamine injections restored all the above parameters to normal values. In conclusion, chlorogenic and caftaric acids before methamphetamine injections prevented liver toxicity and oxidative stress where chlorogenic acid was more effective.

No MeSH data available.


Related in: MedlinePlus