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Chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine.

Koriem KM, Soliman RE - J Toxicol (2014)

Bottom Line: Thirty-two male albino rats were divided into 4 equal groups.Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period.Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Medical Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12622, Egypt ; Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), No. 6 Tingkat 1, Persiaran Seksyen 4/9, Bandar Putra Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.

ABSTRACT
Methamphetamine intoxication can cause acute hepatic failure. Chlorogenic and caftaric acids are the major dietary polyphenols present in various foods. The aim of this study was to evaluate the protective role of chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine in rats. Thirty-two male albino rats were divided into 4 equal groups. Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period. Groups 2, 3, and 4 were injected (i.p) with methamphetamine (10 mg/kg) twice a day over seven-day period, where groups 3 and 4 were injected (i.p) with 60 mg/kg chlorogenic acid and 40 mg/kg caftaric acid, respectively, one day before methamphetamine injections. Methamphetamine increased serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, low-density lipoprotein, and triglycerides. Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels. Chlorogenic and caftaric acids prior to methamphetamine injections restored all the above parameters to normal values. In conclusion, chlorogenic and caftaric acids before methamphetamine injections prevented liver toxicity and oxidative stress where chlorogenic acid was more effective.

No MeSH data available.


Related in: MedlinePlus

(a) The control group with preserved hepatic architecture (H&E ×200). (b) METH injections caused by a hoop of oedema in the periportal area (arrowhead), which compressed the surrounding hepatocytes. The intracytoplasm vacuolation was found (H&E ×400). (c) Pretreatment of chlorogenic acidwith preserved hepatic lobular architecture. The hepatocytes are within normal limit and preserved its plate pattern. Liver almost returns to the normal pattern (H&E ×200). (d) Pretreatment of caftaric acid to METH injected rats with large reserved hepatic lobular architecture and the liver almost returns to the normal pattern (H&E ×200).
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fig1: (a) The control group with preserved hepatic architecture (H&E ×200). (b) METH injections caused by a hoop of oedema in the periportal area (arrowhead), which compressed the surrounding hepatocytes. The intracytoplasm vacuolation was found (H&E ×400). (c) Pretreatment of chlorogenic acidwith preserved hepatic lobular architecture. The hepatocytes are within normal limit and preserved its plate pattern. Liver almost returns to the normal pattern (H&E ×200). (d) Pretreatment of caftaric acid to METH injected rats with large reserved hepatic lobular architecture and the liver almost returns to the normal pattern (H&E ×200).

Mentions: Figure 1 revealed the histopathology results in METH injected rats as well as chlorogenic or caftaric acid potentially pretreated groups. The structure of the control liver showed normal hepatocytes, vascular sinusoids, and centrilobular vein (Figure 1(a)). METH injections caused a hoop of oedema in the periportal area, which compressed the surrounding hepatocytes (Figure 1(b)). Examination of liver sections of rats pretreated with chlorogenic or caftaric acid prior to METH injections showed preserved hepatic lobular architecture. The hepatocytes were within normal limit and preserved its plate pattern. Liver almost returned to the normal pattern (Figures 1(c) and 1(d)).


Chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine.

Koriem KM, Soliman RE - J Toxicol (2014)

(a) The control group with preserved hepatic architecture (H&E ×200). (b) METH injections caused by a hoop of oedema in the periportal area (arrowhead), which compressed the surrounding hepatocytes. The intracytoplasm vacuolation was found (H&E ×400). (c) Pretreatment of chlorogenic acidwith preserved hepatic lobular architecture. The hepatocytes are within normal limit and preserved its plate pattern. Liver almost returns to the normal pattern (H&E ×200). (d) Pretreatment of caftaric acid to METH injected rats with large reserved hepatic lobular architecture and the liver almost returns to the normal pattern (H&E ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127234&req=5

fig1: (a) The control group with preserved hepatic architecture (H&E ×200). (b) METH injections caused by a hoop of oedema in the periportal area (arrowhead), which compressed the surrounding hepatocytes. The intracytoplasm vacuolation was found (H&E ×400). (c) Pretreatment of chlorogenic acidwith preserved hepatic lobular architecture. The hepatocytes are within normal limit and preserved its plate pattern. Liver almost returns to the normal pattern (H&E ×200). (d) Pretreatment of caftaric acid to METH injected rats with large reserved hepatic lobular architecture and the liver almost returns to the normal pattern (H&E ×200).
Mentions: Figure 1 revealed the histopathology results in METH injected rats as well as chlorogenic or caftaric acid potentially pretreated groups. The structure of the control liver showed normal hepatocytes, vascular sinusoids, and centrilobular vein (Figure 1(a)). METH injections caused a hoop of oedema in the periportal area, which compressed the surrounding hepatocytes (Figure 1(b)). Examination of liver sections of rats pretreated with chlorogenic or caftaric acid prior to METH injections showed preserved hepatic lobular architecture. The hepatocytes were within normal limit and preserved its plate pattern. Liver almost returned to the normal pattern (Figures 1(c) and 1(d)).

Bottom Line: Thirty-two male albino rats were divided into 4 equal groups.Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period.Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels.

View Article: PubMed Central - PubMed

Affiliation: Medical Physiology Department, Medical Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12622, Egypt ; Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), No. 6 Tingkat 1, Persiaran Seksyen 4/9, Bandar Putra Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.

ABSTRACT
Methamphetamine intoxication can cause acute hepatic failure. Chlorogenic and caftaric acids are the major dietary polyphenols present in various foods. The aim of this study was to evaluate the protective role of chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine in rats. Thirty-two male albino rats were divided into 4 equal groups. Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period. Groups 2, 3, and 4 were injected (i.p) with methamphetamine (10 mg/kg) twice a day over seven-day period, where groups 3 and 4 were injected (i.p) with 60 mg/kg chlorogenic acid and 40 mg/kg caftaric acid, respectively, one day before methamphetamine injections. Methamphetamine increased serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, low-density lipoprotein, and triglycerides. Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels. Chlorogenic and caftaric acids prior to methamphetamine injections restored all the above parameters to normal values. In conclusion, chlorogenic and caftaric acids before methamphetamine injections prevented liver toxicity and oxidative stress where chlorogenic acid was more effective.

No MeSH data available.


Related in: MedlinePlus