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Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

Beyazyıldız E, Pınarlı FA, Beyazyıldız O, Hekimoğlu ER, Acar U, Demir MN, Albayrak A, Kaymaz F, Sobacı G, Delibaşı T - Stem Cells Int (2014)

Bottom Line: Purpose.Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week.Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, Turkey.

ABSTRACT
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P < 0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy findings of cellular injury observed in PBS-treated rats. Apoptotic cell (arrows) observed in PBS-treated rat cornea (a). Swelling of cells and lytic focuses in cell cytoplasms (thick arrow) were observed. Condensation of mitochondrial matrix, dilatation of smooth endoplasmic reticulum, and decreased secretory granules (thin arrow) were observed in cells (b). Peripheral migration, condensation of chromatins, and irregular expansion of perinuclear cisternas (thick arrow) were observed in nucleus of cells although there were intact desmosomes (thin arrow) between cells (c) (Joel JEM-1400 model, magnification (a), (b): ×5000 and (c): ×12000).
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fig5: Transmission electron microscopy findings of cellular injury observed in PBS-treated rats. Apoptotic cell (arrows) observed in PBS-treated rat cornea (a). Swelling of cells and lytic focuses in cell cytoplasms (thick arrow) were observed. Condensation of mitochondrial matrix, dilatation of smooth endoplasmic reticulum, and decreased secretory granules (thin arrow) were observed in cells (b). Peripheral migration, condensation of chromatins, and irregular expansion of perinuclear cisternas (thick arrow) were observed in nucleus of cells although there were intact desmosomes (thin arrow) between cells (c) (Joel JEM-1400 model, magnification (a), (b): ×5000 and (c): ×12000).

Mentions: Decreased number of goblet cells was observed in conjunctiva of PBS-treated rats and number of secretory granules was decreased in goblet cells of these rats. However, there were increased number of goblet cells in conjunctiva of MSCs-treated rats and these cells contained more secretory granules in their cytoplasm. Meanwhile, there was decreased number of microvilli at apical portions of corneal epithelium of PBS-treated rats, and within the corneas of these rats cells started to show signs of separation from each other and mitochondria of these cells were also enlarged. Signs of cellular injury and apoptotic cells were observed at the cornea epithelium in PBS-treated rats. Microvillus was preserved at apical portions of the corneal epithelium of MSCs-treated rats and there was no prominent sign of cellular injury at cornea of these rats (Figures 4 and 5).


Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

Beyazyıldız E, Pınarlı FA, Beyazyıldız O, Hekimoğlu ER, Acar U, Demir MN, Albayrak A, Kaymaz F, Sobacı G, Delibaşı T - Stem Cells Int (2014)

Transmission electron microscopy findings of cellular injury observed in PBS-treated rats. Apoptotic cell (arrows) observed in PBS-treated rat cornea (a). Swelling of cells and lytic focuses in cell cytoplasms (thick arrow) were observed. Condensation of mitochondrial matrix, dilatation of smooth endoplasmic reticulum, and decreased secretory granules (thin arrow) were observed in cells (b). Peripheral migration, condensation of chromatins, and irregular expansion of perinuclear cisternas (thick arrow) were observed in nucleus of cells although there were intact desmosomes (thin arrow) between cells (c) (Joel JEM-1400 model, magnification (a), (b): ×5000 and (c): ×12000).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127226&req=5

fig5: Transmission electron microscopy findings of cellular injury observed in PBS-treated rats. Apoptotic cell (arrows) observed in PBS-treated rat cornea (a). Swelling of cells and lytic focuses in cell cytoplasms (thick arrow) were observed. Condensation of mitochondrial matrix, dilatation of smooth endoplasmic reticulum, and decreased secretory granules (thin arrow) were observed in cells (b). Peripheral migration, condensation of chromatins, and irregular expansion of perinuclear cisternas (thick arrow) were observed in nucleus of cells although there were intact desmosomes (thin arrow) between cells (c) (Joel JEM-1400 model, magnification (a), (b): ×5000 and (c): ×12000).
Mentions: Decreased number of goblet cells was observed in conjunctiva of PBS-treated rats and number of secretory granules was decreased in goblet cells of these rats. However, there were increased number of goblet cells in conjunctiva of MSCs-treated rats and these cells contained more secretory granules in their cytoplasm. Meanwhile, there was decreased number of microvilli at apical portions of corneal epithelium of PBS-treated rats, and within the corneas of these rats cells started to show signs of separation from each other and mitochondria of these cells were also enlarged. Signs of cellular injury and apoptotic cells were observed at the cornea epithelium in PBS-treated rats. Microvillus was preserved at apical portions of the corneal epithelium of MSCs-treated rats and there was no prominent sign of cellular injury at cornea of these rats (Figures 4 and 5).

Bottom Line: Purpose.Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week.Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, Turkey.

ABSTRACT
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P < 0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

No MeSH data available.


Related in: MedlinePlus