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Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

Beyazyıldız E, Pınarlı FA, Beyazyıldız O, Hekimoğlu ER, Acar U, Demir MN, Albayrak A, Kaymaz F, Sobacı G, Delibaşı T - Stem Cells Int (2014)

Bottom Line: Purpose.Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week.Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, Turkey.

ABSTRACT
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P < 0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

No MeSH data available.


Related in: MedlinePlus

Light microscopy findings. Decreased corneal inflammation was observed in MSCs-treated rat cornea after one week of therapy (a) while diffuse leukocyte infiltration was observed in PBS-treated rat cornea (b). Resolved conjunctival inflammation was observed in MSCs-treated rat conjunctiva after one week of therapy (c) while diffuse leukocyte infiltration and vascular congestion were observed in PBS-treated rat's conjunctiva (d). (H&E staining, magnification: ×200).
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fig3: Light microscopy findings. Decreased corneal inflammation was observed in MSCs-treated rat cornea after one week of therapy (a) while diffuse leukocyte infiltration was observed in PBS-treated rat cornea (b). Resolved conjunctival inflammation was observed in MSCs-treated rat conjunctiva after one week of therapy (c) while diffuse leukocyte infiltration and vascular congestion were observed in PBS-treated rat's conjunctiva (d). (H&E staining, magnification: ×200).

Mentions: There was minimal corneal and conjunctival inflammation in MSCs-treated rats. However, diffuse leukocyte infiltration (primarily, polymorphonuclear leukocyte infiltration) was observed in both the corneas and conjunctiva of PBS-treated rats. In addition, the meibomian glands were also inflamed and congestion of vascular structures was observed in PBS-treated rats (Figure 3).


Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

Beyazyıldız E, Pınarlı FA, Beyazyıldız O, Hekimoğlu ER, Acar U, Demir MN, Albayrak A, Kaymaz F, Sobacı G, Delibaşı T - Stem Cells Int (2014)

Light microscopy findings. Decreased corneal inflammation was observed in MSCs-treated rat cornea after one week of therapy (a) while diffuse leukocyte infiltration was observed in PBS-treated rat cornea (b). Resolved conjunctival inflammation was observed in MSCs-treated rat conjunctiva after one week of therapy (c) while diffuse leukocyte infiltration and vascular congestion were observed in PBS-treated rat's conjunctiva (d). (H&E staining, magnification: ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127226&req=5

fig3: Light microscopy findings. Decreased corneal inflammation was observed in MSCs-treated rat cornea after one week of therapy (a) while diffuse leukocyte infiltration was observed in PBS-treated rat cornea (b). Resolved conjunctival inflammation was observed in MSCs-treated rat conjunctiva after one week of therapy (c) while diffuse leukocyte infiltration and vascular congestion were observed in PBS-treated rat's conjunctiva (d). (H&E staining, magnification: ×200).
Mentions: There was minimal corneal and conjunctival inflammation in MSCs-treated rats. However, diffuse leukocyte infiltration (primarily, polymorphonuclear leukocyte infiltration) was observed in both the corneas and conjunctiva of PBS-treated rats. In addition, the meibomian glands were also inflamed and congestion of vascular structures was observed in PBS-treated rats (Figure 3).

Bottom Line: Purpose.Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week.Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, Turkey.

ABSTRACT
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P < 0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

No MeSH data available.


Related in: MedlinePlus