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Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

Beyazyıldız E, Pınarlı FA, Beyazyıldız O, Hekimoğlu ER, Acar U, Demir MN, Albayrak A, Kaymaz F, Sobacı G, Delibaşı T - Stem Cells Int (2014)

Bottom Line: Purpose.Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week.Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, Turkey.

ABSTRACT
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P < 0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

No MeSH data available.


Related in: MedlinePlus

Representative biomicroscopic fluorescein and Rose Bengal staining photographs of groups. MSC-treated group ((a), (b), and (c), resp.) and PBS-treated group ((d), (e), and (f), resp.). Punctate epitheliopathy was observed in PBS-treated rats and there were increased Rose Bengal and corneal fluorescein score grades in PBS-treated rats.
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fig2: Representative biomicroscopic fluorescein and Rose Bengal staining photographs of groups. MSC-treated group ((a), (b), and (c), resp.) and PBS-treated group ((d), (e), and (f), resp.). Punctate epitheliopathy was observed in PBS-treated rats and there were increased Rose Bengal and corneal fluorescein score grades in PBS-treated rats.

Mentions: Mean aqueous tear volume and BUT scores of MSCs-treated group significantly increased (P = 0.007 and P = 0.005, resp.) and were significantly higher than those of PBS-treated group after one week of therapy (P = 0.04 and P = 0.003, resp.). Corneal epithelial fluorescein and Rose Bengal scores of MSCs-treated group were significantly decreased (P = 0.006 and P = 0.01, resp.) after one week of therapy. Rose Bengal scores after one week of MSCs therapy were significantly lower than PBS-treated group scores (P = 0.001) and corneal fluorescein scores were lower than PBS-treated group scores, not significantly (P = 0.07) (Figures 1 and 2).


Efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model.

Beyazyıldız E, Pınarlı FA, Beyazyıldız O, Hekimoğlu ER, Acar U, Demir MN, Albayrak A, Kaymaz F, Sobacı G, Delibaşı T - Stem Cells Int (2014)

Representative biomicroscopic fluorescein and Rose Bengal staining photographs of groups. MSC-treated group ((a), (b), and (c), resp.) and PBS-treated group ((d), (e), and (f), resp.). Punctate epitheliopathy was observed in PBS-treated rats and there were increased Rose Bengal and corneal fluorescein score grades in PBS-treated rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127226&req=5

fig2: Representative biomicroscopic fluorescein and Rose Bengal staining photographs of groups. MSC-treated group ((a), (b), and (c), resp.) and PBS-treated group ((d), (e), and (f), resp.). Punctate epitheliopathy was observed in PBS-treated rats and there were increased Rose Bengal and corneal fluorescein score grades in PBS-treated rats.
Mentions: Mean aqueous tear volume and BUT scores of MSCs-treated group significantly increased (P = 0.007 and P = 0.005, resp.) and were significantly higher than those of PBS-treated group after one week of therapy (P = 0.04 and P = 0.003, resp.). Corneal epithelial fluorescein and Rose Bengal scores of MSCs-treated group were significantly decreased (P = 0.006 and P = 0.01, resp.) after one week of therapy. Rose Bengal scores after one week of MSCs therapy were significantly lower than PBS-treated group scores (P = 0.001) and corneal fluorescein scores were lower than PBS-treated group scores, not significantly (P = 0.07) (Figures 1 and 2).

Bottom Line: Purpose.Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week.Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, Turkey.

ABSTRACT
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n = 9) or phosphate buffer solution (n = 7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P < 0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

No MeSH data available.


Related in: MedlinePlus