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Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

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Related in: MedlinePlus

Scanning electron micrograph showing abaxial (a) and adaxial (c) surfaces of in vitro leaves of plantlet of Calathea crotalifera, abaxial (b) and adaxial (d) surfaces of leaves from in vivo (acclimatized) plant.
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fig5: Scanning electron micrograph showing abaxial (a) and adaxial (c) surfaces of in vitro leaves of plantlet of Calathea crotalifera, abaxial (b) and adaxial (d) surfaces of leaves from in vivo (acclimatized) plant.

Mentions: The most crucial phase in micropropagation study is acclimatization of complete in vitro plantlets to the natural environment. Healthy plantlets taken out from the in vitro condition usually produced leaves with low epicuticular wax and with ineffective control of stomatal function [52]. These phenomena promote water loss through transpiration when the plantlets are transferred to the soil. In the present study, high quality of in vitro plantlets of C. crotalifera was successfully acclimatized with 75% of plantlet survival after four weeks of transfer (Figure 4(i)). In general, there were no morphological differences in the vegetative characters between in vivo and in vitro plant of C. crotalifera except for the sizes and texture of leaves. The texture of in vitro leaves surface was membranous while in vivo leaves were coriaceous due to the presence of thick cuticle layer with the presence of wax on in vivo leaves and absence of it on in vitro leaves as shown in Figure 5 under scanning electron microscope. Based on this micromorphological study, the acclimatized plantlets were well developed to adapt with the natural environment since they were exposed to the sun. The presence and thickness of the cuticle is dictated by environmental factor including sunlight and can be an indicator for climate and habitat [53]. The difference of cuticle thickness between in vitro and in vivo leaves has been reported in acclimatization of micropropagated of Murraya paniculata plantlets [54]. The leaf lamina of in vitro and in vivo of C. crotalifera also showed little differences on anatomical characters since both leaves were hypostomatic. The mean number of stomata was higher on the abaxial surface compared to adaxial surface of the leaves (Table 9). This anatomical characteristic was also found on C. orbifolia (Linden) leaves as reported by Yang and Yeh [1].


Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Scanning electron micrograph showing abaxial (a) and adaxial (c) surfaces of in vitro leaves of plantlet of Calathea crotalifera, abaxial (b) and adaxial (d) surfaces of leaves from in vivo (acclimatized) plant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127217&req=5

fig5: Scanning electron micrograph showing abaxial (a) and adaxial (c) surfaces of in vitro leaves of plantlet of Calathea crotalifera, abaxial (b) and adaxial (d) surfaces of leaves from in vivo (acclimatized) plant.
Mentions: The most crucial phase in micropropagation study is acclimatization of complete in vitro plantlets to the natural environment. Healthy plantlets taken out from the in vitro condition usually produced leaves with low epicuticular wax and with ineffective control of stomatal function [52]. These phenomena promote water loss through transpiration when the plantlets are transferred to the soil. In the present study, high quality of in vitro plantlets of C. crotalifera was successfully acclimatized with 75% of plantlet survival after four weeks of transfer (Figure 4(i)). In general, there were no morphological differences in the vegetative characters between in vivo and in vitro plant of C. crotalifera except for the sizes and texture of leaves. The texture of in vitro leaves surface was membranous while in vivo leaves were coriaceous due to the presence of thick cuticle layer with the presence of wax on in vivo leaves and absence of it on in vitro leaves as shown in Figure 5 under scanning electron microscope. Based on this micromorphological study, the acclimatized plantlets were well developed to adapt with the natural environment since they were exposed to the sun. The presence and thickness of the cuticle is dictated by environmental factor including sunlight and can be an indicator for climate and habitat [53]. The difference of cuticle thickness between in vitro and in vivo leaves has been reported in acclimatization of micropropagated of Murraya paniculata plantlets [54]. The leaf lamina of in vitro and in vivo of C. crotalifera also showed little differences on anatomical characters since both leaves were hypostomatic. The mean number of stomata was higher on the abaxial surface compared to adaxial surface of the leaves (Table 9). This anatomical characteristic was also found on C. orbifolia (Linden) leaves as reported by Yang and Yeh [1].

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Show MeSH
Related in: MedlinePlus