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Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

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Related in: MedlinePlus

Effects of medium solidification and apex damage. (a) Swollenness of explant in media with 3 g/L plant agar. (b) Leaves show vitrification problem in media with 3 g/L plant agar, (c) regenerated shoots with necrotic leaves in media with 8 g/L plant agar. (d), (e), and (f) Induction of microshoots through elimination of apical dominance.
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Related In: Results  -  Collection


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fig3: Effects of medium solidification and apex damage. (a) Swollenness of explant in media with 3 g/L plant agar. (b) Leaves show vitrification problem in media with 3 g/L plant agar, (c) regenerated shoots with necrotic leaves in media with 8 g/L plant agar. (d), (e), and (f) Induction of microshoots through elimination of apical dominance.

Mentions: The using of different concentrations of agar (0.3%, 0.6%, and 0.8%) had showed significant effects on shoot production as shown in Table 2 after eight weeks of culture. The number and height of shoot produced in media augmented with 3.0 g/L and 8.0 g/L g of plant agar was lower for both species of C. crotalifera. Development of the shoots produced in these respective media was not in good condition which showed vitrification and browning problem as shown in Figure 3. The best concentration of plant agar for shoot induction in the present study was 6.0 g/L and this concentration was used in the next multiplication media. The similar application has been reported in induction of shoot of Maranta leuconeura cv. Kerchoviana on medium augmented with 6 g/L plant agar [6]. Vitrified leaves observed in the present study were caused by high relative humidity [15] and can be reduced using high concentration of gelling agents [16]. Kataeva et al. [17] reported that the vitrification problem has been correlated to water availability, microelements, and hormonal imbalance in the tissue culture medium. Vitrification or well known as hyperhydricity is a morphological, anatomical, and physiological malformation that makes the plant tissue water-swollen [18] and the in vitro plantlets will have poor epicuticular wax production [19].


Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Effects of medium solidification and apex damage. (a) Swollenness of explant in media with 3 g/L plant agar. (b) Leaves show vitrification problem in media with 3 g/L plant agar, (c) regenerated shoots with necrotic leaves in media with 8 g/L plant agar. (d), (e), and (f) Induction of microshoots through elimination of apical dominance.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127217&req=5

fig3: Effects of medium solidification and apex damage. (a) Swollenness of explant in media with 3 g/L plant agar. (b) Leaves show vitrification problem in media with 3 g/L plant agar, (c) regenerated shoots with necrotic leaves in media with 8 g/L plant agar. (d), (e), and (f) Induction of microshoots through elimination of apical dominance.
Mentions: The using of different concentrations of agar (0.3%, 0.6%, and 0.8%) had showed significant effects on shoot production as shown in Table 2 after eight weeks of culture. The number and height of shoot produced in media augmented with 3.0 g/L and 8.0 g/L g of plant agar was lower for both species of C. crotalifera. Development of the shoots produced in these respective media was not in good condition which showed vitrification and browning problem as shown in Figure 3. The best concentration of plant agar for shoot induction in the present study was 6.0 g/L and this concentration was used in the next multiplication media. The similar application has been reported in induction of shoot of Maranta leuconeura cv. Kerchoviana on medium augmented with 6 g/L plant agar [6]. Vitrified leaves observed in the present study were caused by high relative humidity [15] and can be reduced using high concentration of gelling agents [16]. Kataeva et al. [17] reported that the vitrification problem has been correlated to water availability, microelements, and hormonal imbalance in the tissue culture medium. Vitrification or well known as hyperhydricity is a morphological, anatomical, and physiological malformation that makes the plant tissue water-swollen [18] and the in vitro plantlets will have poor epicuticular wax production [19].

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Show MeSH
Related in: MedlinePlus