Limits...
Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Show MeSH

Related in: MedlinePlus

Percentage of surviving explants under different treatments in surface sterilization.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4127217&req=5

fig2: Percentage of surviving explants under different treatments in surface sterilization.

Mentions: Two different types of young shoot bud explants were used in this study, which are apical shoot bud from soil medium and axillary shoot bud from soilless medium. Both of these explants were surface sterilized using the same protocol based on Figure 1 and Table 1, respectively. In the present study, axillary shoot buds showed the lowest percentage of contamination (25%) when compared to apical shoot buds (60%). Thus, comparison of the effectiveness in three different surface sterilization treatments (T1, T2, and T3) on axillary shoot bud explants was used in this study. The best treatment of surface sterilization was achieved when 30% NaOCl, 70% ethanol, and 0.3% HgCl2 were utilized in T3 with the lowest percentage of contamination (25 ± 5%) compared to T1 and T2. Furthermore, percentage of surviving explants was higher in T3 (90 ± 6%) followed by T2 and T1 even though the percentage of necrotic leaf in T3 is higher compared to other treatments. Most of the contamination is caused by bacteria and no fungi infections were found. Establishment of contamination free cultures was a major task since the explants were taken from the underground rhizomes [12]. The using of axillary bud that initially sprouted in soil free condition can actually reduce the potential of the explants to be infected by the soil bacteria. This method has been used widely in micropropagation of other rhizomatic species like Heliconia psittacorum [9], Boesenbergia rotunda L. [13], and Curcuma mangga [7]. Disinfecting with 70% ethanol prior to soaking in HgCl2 enhances the contact between HgCl2 and the surface of explants efficiently. Smith [14] reported that mercury ions in HgCl2 solution can break the structure of the cell membrane and the cytoplasm constituents of pathogenic microorganisms by interfering the enzymes and also the protein molecules. The concentration of HgCl2 was not increased more than 0.3% as in T3 because the high concentration of HgCl2 is phytotoxic to plant cells [7]. Based on the results, T3 was the most effective method with the highest survival rate (Figure 2) after four weeks of cultured. This treatment was therefore applied for subsequent experiments.


Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Percentage of surviving explants under different treatments in surface sterilization.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127217&req=5

fig2: Percentage of surviving explants under different treatments in surface sterilization.
Mentions: Two different types of young shoot bud explants were used in this study, which are apical shoot bud from soil medium and axillary shoot bud from soilless medium. Both of these explants were surface sterilized using the same protocol based on Figure 1 and Table 1, respectively. In the present study, axillary shoot buds showed the lowest percentage of contamination (25%) when compared to apical shoot buds (60%). Thus, comparison of the effectiveness in three different surface sterilization treatments (T1, T2, and T3) on axillary shoot bud explants was used in this study. The best treatment of surface sterilization was achieved when 30% NaOCl, 70% ethanol, and 0.3% HgCl2 were utilized in T3 with the lowest percentage of contamination (25 ± 5%) compared to T1 and T2. Furthermore, percentage of surviving explants was higher in T3 (90 ± 6%) followed by T2 and T1 even though the percentage of necrotic leaf in T3 is higher compared to other treatments. Most of the contamination is caused by bacteria and no fungi infections were found. Establishment of contamination free cultures was a major task since the explants were taken from the underground rhizomes [12]. The using of axillary bud that initially sprouted in soil free condition can actually reduce the potential of the explants to be infected by the soil bacteria. This method has been used widely in micropropagation of other rhizomatic species like Heliconia psittacorum [9], Boesenbergia rotunda L. [13], and Curcuma mangga [7]. Disinfecting with 70% ethanol prior to soaking in HgCl2 enhances the contact between HgCl2 and the surface of explants efficiently. Smith [14] reported that mercury ions in HgCl2 solution can break the structure of the cell membrane and the cytoplasm constituents of pathogenic microorganisms by interfering the enzymes and also the protein molecules. The concentration of HgCl2 was not increased more than 0.3% as in T3 because the high concentration of HgCl2 is phytotoxic to plant cells [7]. Based on the results, T3 was the most effective method with the highest survival rate (Figure 2) after four weeks of cultured. This treatment was therefore applied for subsequent experiments.

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Show MeSH
Related in: MedlinePlus