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Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

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Related in: MedlinePlus

General sterilization method for shoot bud explants of Calathea crotalifera.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: General sterilization method for shoot bud explants of Calathea crotalifera.

Mentions: Mature plants of red bract C. Crotalifera (CCR) and yellow bract C. Crotalifera (CCY) were purchased from local farm, Agrobiosolution Company located in Kuantan, Pahang, Malaysia. The plants were maintained at planting area at Centre for Foundation Studies in Science, University of Malaya, under natural condition. Two different sources of young shoot buds were used as explants in this study. The first source of explants is apical young shoot buds obtained directly from the mature plants planted in the soil. The second source of explants was obtained from the sprouting axillary young shoot buds that appeared from the cleaned rhizomes after two weeks under soil free conditions. Suitable explants were collected and washed with detergent under running tap water to remove any soil particles attached to the shoot buds. The external leaves were removed and the shoot buds were trimmed down until the size ranged from 2.5 to 3.0 cm. The explants were then rinsed under running tap water for 30 minutes. Under aseptic conditions, the explants were surface sterilized with three different treatments as shown in Table 1 by following the general sterilization method summarized in Figure 1. Each disinfectant (30% sodium hypochlorite, NaOCl, 70% ethanol, and mercury chloride, HgCl2) was added together with two to three drops of Tween-20 to reduce surface tension. The surface sterilized explants had their external leaves removed and were finally dried on filter papers in the laminar flow air chamber prior to inoculation on media. The contamination, necrotic, and survival percentages of cultures were determined as in Sundram [7].


Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

Rozali SE, Rashid KA, Taha RM - ScientificWorldJournal (2014)

General sterilization method for shoot bud explants of Calathea crotalifera.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4127217&req=5

fig1: General sterilization method for shoot bud explants of Calathea crotalifera.
Mentions: Mature plants of red bract C. Crotalifera (CCR) and yellow bract C. Crotalifera (CCY) were purchased from local farm, Agrobiosolution Company located in Kuantan, Pahang, Malaysia. The plants were maintained at planting area at Centre for Foundation Studies in Science, University of Malaya, under natural condition. Two different sources of young shoot buds were used as explants in this study. The first source of explants is apical young shoot buds obtained directly from the mature plants planted in the soil. The second source of explants was obtained from the sprouting axillary young shoot buds that appeared from the cleaned rhizomes after two weeks under soil free conditions. Suitable explants were collected and washed with detergent under running tap water to remove any soil particles attached to the shoot buds. The external leaves were removed and the shoot buds were trimmed down until the size ranged from 2.5 to 3.0 cm. The explants were then rinsed under running tap water for 30 minutes. Under aseptic conditions, the explants were surface sterilized with three different treatments as shown in Table 1 by following the general sterilization method summarized in Figure 1. Each disinfectant (30% sodium hypochlorite, NaOCl, 70% ethanol, and mercury chloride, HgCl2) was added together with two to three drops of Tween-20 to reduce surface tension. The surface sterilized explants had their external leaves removed and were finally dried on filter papers in the laminar flow air chamber prior to inoculation on media. The contamination, necrotic, and survival percentages of cultures were determined as in Sundram [7].

Bottom Line: A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera.Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem.This is the first report of rapid mass propagation for C. crotalifera.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

Show MeSH
Related in: MedlinePlus