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Isolation and complete nucleotide sequence of a Batai virus strain in Inner Mongolia, China.

Liu H, Shao XQ, Hu B, Zhao JJ, Zhang L, Zhang HL, Bai X, Zhang RX, Niu DY, Sun YG, Yan XJ - Virol. J. (2014)

Bottom Line: Phylogenetic analyses based on full-length genomic sequences indicated that the M and L segments, and a portion of the S segment, of NM/12 are most closely related to the BATV strains isolated in Asia.The S and M segments of NM/12 were independent of phylogenetic lineages.The L segment was the most closely related to Chittoor/IG-20217 (isolated in India), and distantly related to isolated strains in Italy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Zoonoses, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences CAAS, 15 Luming Street, Jilin 132109, China. tcsyxj@126.com.

ABSTRACT

Background: Batai virus (BATV) is a member of the Orthobunyavirus genus of the family Bunyaviridae, and a vector-borne pathogen. Genomic variations of BATV exist in different regions of the world, due to genetic reassortment. Whole-genome sequencing of any isolate is necessary for a phylogenetic analysis. In 1998, a BATV strain was isolated from an Anopheles philippines mosquito in Yunnan Province, China. This strain has not been found to infect any other host. We investigated BATV infection in cattle in Inner Mongolia, China and performed deep sequencing of the genome of the BATV isolate.

Findings: Ninety-five blood samples were collected from cattle in Inner Mongolia, China in 2012. The BATV infection rate was 2.1%. Previously, BATV strain NM/12 was isolated from two cattle in Inner Mongolia, China, and the whole genomic sequence of the strain has been available. We determined the complete genomic nucleotide sequences of the small (S), medium (M), and large (L) genome segments using bovine blood obtained in 2012, and the nucleotide homologies of these segments with those from GenBank were 88.7%-97%, 84%-95.4%, and 72.6%-95.8%, respectively. The deduced amino acid identities were 87.2-99.7%, 64.2-96.8%, and 81.1-98.6%. Phylogenetic analyses based on full-length genomic sequences indicated that the M and L segments, and a portion of the S segment, of NM/12 are most closely related to the BATV strains isolated in Asia. The S and M segments of NM/12 were independent of phylogenetic lineages. The L segment was the most closely related to Chittoor/IG-20217 (isolated in India), and distantly related to isolated strains in Italy. Nucleotide substitution rates in the nucleotide sequences that code for the nucleocapsid, envelope glycoprotein, and polymerase protein of NM/12 strain were 2.56%, 4.69%, and 4.21%, respectively, relative to the original strain of MM2222.

Conclusion: A novel BATV NM/12 strain from bovine serum collected in Inner Mongolia was isolated from cattle in China for the first time. Our findings elucidate the evolutionary status of the BATV NM/12 strain among different orthobunyavirus strains and may provide some clues to prevent the emergence of BATV infection in cattle and humans.

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Electron microscopy of negative-stained Batai virus particles from cattle. Scale bar indicates 100 nm.
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Figure 2: Electron microscopy of negative-stained Batai virus particles from cattle. Scale bar indicates 100 nm.

Mentions: At 72 h post-inoculation the suspensions of febrile cattle brain tissue and Vero cell cultures showed a distinct CPE characterized by cell rounding, pyknosis, ‘Fleece-Pulling’ to the thyrsoid and cell monolayer destruction (data not shown). TEM of negatively stained samples revealed medium-sized particles with a diameter of approximately 50 nm within an envelope, and characteristics of typical of BATV (Figure 2). RT-PCR revealed that the supernatants of cultures were positive only for BATV (Figure 1). The positive PCR products of the M gene from the brain tissue suspensions and cell cultures were identified by sequence analysis. The 550-bp sequence of the M gene from BATV from the suspensions shared 100% homology with that of Vero isolates.


Isolation and complete nucleotide sequence of a Batai virus strain in Inner Mongolia, China.

Liu H, Shao XQ, Hu B, Zhao JJ, Zhang L, Zhang HL, Bai X, Zhang RX, Niu DY, Sun YG, Yan XJ - Virol. J. (2014)

Electron microscopy of negative-stained Batai virus particles from cattle. Scale bar indicates 100 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4127039&req=5

Figure 2: Electron microscopy of negative-stained Batai virus particles from cattle. Scale bar indicates 100 nm.
Mentions: At 72 h post-inoculation the suspensions of febrile cattle brain tissue and Vero cell cultures showed a distinct CPE characterized by cell rounding, pyknosis, ‘Fleece-Pulling’ to the thyrsoid and cell monolayer destruction (data not shown). TEM of negatively stained samples revealed medium-sized particles with a diameter of approximately 50 nm within an envelope, and characteristics of typical of BATV (Figure 2). RT-PCR revealed that the supernatants of cultures were positive only for BATV (Figure 1). The positive PCR products of the M gene from the brain tissue suspensions and cell cultures were identified by sequence analysis. The 550-bp sequence of the M gene from BATV from the suspensions shared 100% homology with that of Vero isolates.

Bottom Line: Phylogenetic analyses based on full-length genomic sequences indicated that the M and L segments, and a portion of the S segment, of NM/12 are most closely related to the BATV strains isolated in Asia.The S and M segments of NM/12 were independent of phylogenetic lineages.The L segment was the most closely related to Chittoor/IG-20217 (isolated in India), and distantly related to isolated strains in Italy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Zoonoses, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences CAAS, 15 Luming Street, Jilin 132109, China. tcsyxj@126.com.

ABSTRACT

Background: Batai virus (BATV) is a member of the Orthobunyavirus genus of the family Bunyaviridae, and a vector-borne pathogen. Genomic variations of BATV exist in different regions of the world, due to genetic reassortment. Whole-genome sequencing of any isolate is necessary for a phylogenetic analysis. In 1998, a BATV strain was isolated from an Anopheles philippines mosquito in Yunnan Province, China. This strain has not been found to infect any other host. We investigated BATV infection in cattle in Inner Mongolia, China and performed deep sequencing of the genome of the BATV isolate.

Findings: Ninety-five blood samples were collected from cattle in Inner Mongolia, China in 2012. The BATV infection rate was 2.1%. Previously, BATV strain NM/12 was isolated from two cattle in Inner Mongolia, China, and the whole genomic sequence of the strain has been available. We determined the complete genomic nucleotide sequences of the small (S), medium (M), and large (L) genome segments using bovine blood obtained in 2012, and the nucleotide homologies of these segments with those from GenBank were 88.7%-97%, 84%-95.4%, and 72.6%-95.8%, respectively. The deduced amino acid identities were 87.2-99.7%, 64.2-96.8%, and 81.1-98.6%. Phylogenetic analyses based on full-length genomic sequences indicated that the M and L segments, and a portion of the S segment, of NM/12 are most closely related to the BATV strains isolated in Asia. The S and M segments of NM/12 were independent of phylogenetic lineages. The L segment was the most closely related to Chittoor/IG-20217 (isolated in India), and distantly related to isolated strains in Italy. Nucleotide substitution rates in the nucleotide sequences that code for the nucleocapsid, envelope glycoprotein, and polymerase protein of NM/12 strain were 2.56%, 4.69%, and 4.21%, respectively, relative to the original strain of MM2222.

Conclusion: A novel BATV NM/12 strain from bovine serum collected in Inner Mongolia was isolated from cattle in China for the first time. Our findings elucidate the evolutionary status of the BATV NM/12 strain among different orthobunyavirus strains and may provide some clues to prevent the emergence of BATV infection in cattle and humans.

Show MeSH
Related in: MedlinePlus