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Elevated CXCL-8 expression in bronchoalveolar lavage correlates with disease severity in patients with acute respiratory distress syndrome resulting from tuberculosis.

Hashemian SM, Mortaz E, Tabarsi P, Jamaati H, Maghsoomi Z, Khosravi A, Garssen J, Masjedi MR, Velayati AA, Folkerts G, Barnes PJ, Adcock IM - J Inflamm (Lond) (2014)

Bottom Line: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups.In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB.This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Tuberculosis (TB) is a rare but known cause of acute respiratory distress syndrome (ARDS). The role of inflammatory cytokines in the progression of ARDS in TB patients is unknown.

Objectives: In this study we investigated the possible link between the levels of inflammatory cytokines in bronchoalveolar lavage (BAL) in patients with TB or ARDS alone or in patients with TB-induced ARDS (ARDS + TB).

Methods: 90 patients were studied: 30 with TB alone, 30 with ARDS alone and 30 with ARDS + TB. BAL was collected by fiberoptic bronchoscopy and the concentrations of interleukin(IL)-6, CXCL8, TNF-α and IL-1β and the amounts of total protein were measured by ELISA and bicinchoninic acid assay (BCA) methods respectively. The correlation between disease severity measured by Murray scores, SOFA and APACHE II analysis and BAL mediators and cells was also determined.

Results: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups. Disease severity in the ARDS + TB group as determined by Murray score correlated with BAL CXCL8 and neutrophils but not with IL-6, IL-1β and TNF-α concentrations. In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB. This difference in CXCL8 was lost in the presence of ARDS.

Conclusions: BAL CXCL8 levels were significantly higher in patients with ARDS induced by TB and could suggest an important role of CXCL8 in the pathogenesis of this form of ARDS. This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

No MeSH data available.


Related in: MedlinePlus

Correlations between CXCL8 levels in bronchoalveolar lavage (BAL) and patient mortality. BAL CXCL8 levels were correlated with Acute Physiology and Chronic Health Evaluation (APACHE II) (panels A, B & C), Murray (panels D, E & F) and Sequential Organ Failure Assessment (SOFA) (panels G, H & I) scores in patients with Tuberculosis (TB) (panels A, D & G), Acute Respiratory Distress Syndrome (ARDS) (panels B, E & H) and the combination of both TB and ARDS (panels C, F and I). Data were analysed using Pearson’s correlation of coefficient. A p value of 0.05 or less is considered significant.
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Figure 5: Correlations between CXCL8 levels in bronchoalveolar lavage (BAL) and patient mortality. BAL CXCL8 levels were correlated with Acute Physiology and Chronic Health Evaluation (APACHE II) (panels A, B & C), Murray (panels D, E & F) and Sequential Organ Failure Assessment (SOFA) (panels G, H & I) scores in patients with Tuberculosis (TB) (panels A, D & G), Acute Respiratory Distress Syndrome (ARDS) (panels B, E & H) and the combination of both TB and ARDS (panels C, F and I). Data were analysed using Pearson’s correlation of coefficient. A p value of 0.05 or less is considered significant.

Mentions: There was a significant correlation between BAL CXCL-8 levels and BAL neutrophilia but this was not maintained within the miliary TB and non-miliary groups. BAL CXCL-8 levels significantly correlated with disease severity as measured by APACHE II, Murray and SOFA scores in patients with ARDS whether they had TB or not (Figure 4). In contrast, BAL CXCL-8 levels only correlated significantly with the SOFA score in patients with TB alone (Figure 5). There was no correlation between the BAL levels of IL-6, IL-1β and TNF-α and the APACHE II, Murray or SOFA scores in any of the patients groups (data not shown).


Elevated CXCL-8 expression in bronchoalveolar lavage correlates with disease severity in patients with acute respiratory distress syndrome resulting from tuberculosis.

Hashemian SM, Mortaz E, Tabarsi P, Jamaati H, Maghsoomi Z, Khosravi A, Garssen J, Masjedi MR, Velayati AA, Folkerts G, Barnes PJ, Adcock IM - J Inflamm (Lond) (2014)

Correlations between CXCL8 levels in bronchoalveolar lavage (BAL) and patient mortality. BAL CXCL8 levels were correlated with Acute Physiology and Chronic Health Evaluation (APACHE II) (panels A, B & C), Murray (panels D, E & F) and Sequential Organ Failure Assessment (SOFA) (panels G, H & I) scores in patients with Tuberculosis (TB) (panels A, D & G), Acute Respiratory Distress Syndrome (ARDS) (panels B, E & H) and the combination of both TB and ARDS (panels C, F and I). Data were analysed using Pearson’s correlation of coefficient. A p value of 0.05 or less is considered significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4126912&req=5

Figure 5: Correlations between CXCL8 levels in bronchoalveolar lavage (BAL) and patient mortality. BAL CXCL8 levels were correlated with Acute Physiology and Chronic Health Evaluation (APACHE II) (panels A, B & C), Murray (panels D, E & F) and Sequential Organ Failure Assessment (SOFA) (panels G, H & I) scores in patients with Tuberculosis (TB) (panels A, D & G), Acute Respiratory Distress Syndrome (ARDS) (panels B, E & H) and the combination of both TB and ARDS (panels C, F and I). Data were analysed using Pearson’s correlation of coefficient. A p value of 0.05 or less is considered significant.
Mentions: There was a significant correlation between BAL CXCL-8 levels and BAL neutrophilia but this was not maintained within the miliary TB and non-miliary groups. BAL CXCL-8 levels significantly correlated with disease severity as measured by APACHE II, Murray and SOFA scores in patients with ARDS whether they had TB or not (Figure 4). In contrast, BAL CXCL-8 levels only correlated significantly with the SOFA score in patients with TB alone (Figure 5). There was no correlation between the BAL levels of IL-6, IL-1β and TNF-α and the APACHE II, Murray or SOFA scores in any of the patients groups (data not shown).

Bottom Line: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups.In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB.This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Tuberculosis (TB) is a rare but known cause of acute respiratory distress syndrome (ARDS). The role of inflammatory cytokines in the progression of ARDS in TB patients is unknown.

Objectives: In this study we investigated the possible link between the levels of inflammatory cytokines in bronchoalveolar lavage (BAL) in patients with TB or ARDS alone or in patients with TB-induced ARDS (ARDS + TB).

Methods: 90 patients were studied: 30 with TB alone, 30 with ARDS alone and 30 with ARDS + TB. BAL was collected by fiberoptic bronchoscopy and the concentrations of interleukin(IL)-6, CXCL8, TNF-α and IL-1β and the amounts of total protein were measured by ELISA and bicinchoninic acid assay (BCA) methods respectively. The correlation between disease severity measured by Murray scores, SOFA and APACHE II analysis and BAL mediators and cells was also determined.

Results: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups. Disease severity in the ARDS + TB group as determined by Murray score correlated with BAL CXCL8 and neutrophils but not with IL-6, IL-1β and TNF-α concentrations. In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB. This difference in CXCL8 was lost in the presence of ARDS.

Conclusions: BAL CXCL8 levels were significantly higher in patients with ARDS induced by TB and could suggest an important role of CXCL8 in the pathogenesis of this form of ARDS. This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

No MeSH data available.


Related in: MedlinePlus