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Elevated CXCL-8 expression in bronchoalveolar lavage correlates with disease severity in patients with acute respiratory distress syndrome resulting from tuberculosis.

Hashemian SM, Mortaz E, Tabarsi P, Jamaati H, Maghsoomi Z, Khosravi A, Garssen J, Masjedi MR, Velayati AA, Folkerts G, Barnes PJ, Adcock IM - J Inflamm (Lond) (2014)

Bottom Line: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups.In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB.This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Tuberculosis (TB) is a rare but known cause of acute respiratory distress syndrome (ARDS). The role of inflammatory cytokines in the progression of ARDS in TB patients is unknown.

Objectives: In this study we investigated the possible link between the levels of inflammatory cytokines in bronchoalveolar lavage (BAL) in patients with TB or ARDS alone or in patients with TB-induced ARDS (ARDS + TB).

Methods: 90 patients were studied: 30 with TB alone, 30 with ARDS alone and 30 with ARDS + TB. BAL was collected by fiberoptic bronchoscopy and the concentrations of interleukin(IL)-6, CXCL8, TNF-α and IL-1β and the amounts of total protein were measured by ELISA and bicinchoninic acid assay (BCA) methods respectively. The correlation between disease severity measured by Murray scores, SOFA and APACHE II analysis and BAL mediators and cells was also determined.

Results: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups. Disease severity in the ARDS + TB group as determined by Murray score correlated with BAL CXCL8 and neutrophils but not with IL-6, IL-1β and TNF-α concentrations. In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB. This difference in CXCL8 was lost in the presence of ARDS.

Conclusions: BAL CXCL8 levels were significantly higher in patients with ARDS induced by TB and could suggest an important role of CXCL8 in the pathogenesis of this form of ARDS. This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

No MeSH data available.


Related in: MedlinePlus

Differential cell counts in bronchoalveolar lavage (BAL) of patients with tuberculosis (TB), acute respiratory distress syndrome (ARDS) and the combination of both TB and ARDS. Cytospins of BAL cells were distinguished by diff Quick staining and by morphology (Hemacolor, original magnifi cation x 1,000) (A). Total cells were counted and the percentage of each cell type was calculated when the counted total cell numbers were more than 200 and represented as neutrophils, macrophages and lymphocytes (B). The data among groups were analyzed using one-way analysis of variance, and the differences between two groups were tested using an unpaired t test; P ≤ .05 was considered significant. Data are presented as mean ± SEM (n = 30 in each group). *p ≤ 0.05 between groups.
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Figure 3: Differential cell counts in bronchoalveolar lavage (BAL) of patients with tuberculosis (TB), acute respiratory distress syndrome (ARDS) and the combination of both TB and ARDS. Cytospins of BAL cells were distinguished by diff Quick staining and by morphology (Hemacolor, original magnifi cation x 1,000) (A). Total cells were counted and the percentage of each cell type was calculated when the counted total cell numbers were more than 200 and represented as neutrophils, macrophages and lymphocytes (B). The data among groups were analyzed using one-way analysis of variance, and the differences between two groups were tested using an unpaired t test; P ≤ .05 was considered significant. Data are presented as mean ± SEM (n = 30 in each group). *p ≤ 0.05 between groups.

Mentions: Differential cell counts were performed on cytospins obtained from BAL fluid. In BAL, the most abundant cells observed in the ARDS, TB and TB + ARDS groups were neutrophils (Figure 3A) which comprised 67.8 ± 5.8%, 78.2 ± 3.0 and 91 ± 4.4% of the total cells in each sample, respectively (Figure 3B). The numbers of BAL neutrophils were significantly greater between TB alone and ARDS alone groups (P < 0.05) and between the TB alone and ARDS + TB groups.


Elevated CXCL-8 expression in bronchoalveolar lavage correlates with disease severity in patients with acute respiratory distress syndrome resulting from tuberculosis.

Hashemian SM, Mortaz E, Tabarsi P, Jamaati H, Maghsoomi Z, Khosravi A, Garssen J, Masjedi MR, Velayati AA, Folkerts G, Barnes PJ, Adcock IM - J Inflamm (Lond) (2014)

Differential cell counts in bronchoalveolar lavage (BAL) of patients with tuberculosis (TB), acute respiratory distress syndrome (ARDS) and the combination of both TB and ARDS. Cytospins of BAL cells were distinguished by diff Quick staining and by morphology (Hemacolor, original magnifi cation x 1,000) (A). Total cells were counted and the percentage of each cell type was calculated when the counted total cell numbers were more than 200 and represented as neutrophils, macrophages and lymphocytes (B). The data among groups were analyzed using one-way analysis of variance, and the differences between two groups were tested using an unpaired t test; P ≤ .05 was considered significant. Data are presented as mean ± SEM (n = 30 in each group). *p ≤ 0.05 between groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4126912&req=5

Figure 3: Differential cell counts in bronchoalveolar lavage (BAL) of patients with tuberculosis (TB), acute respiratory distress syndrome (ARDS) and the combination of both TB and ARDS. Cytospins of BAL cells were distinguished by diff Quick staining and by morphology (Hemacolor, original magnifi cation x 1,000) (A). Total cells were counted and the percentage of each cell type was calculated when the counted total cell numbers were more than 200 and represented as neutrophils, macrophages and lymphocytes (B). The data among groups were analyzed using one-way analysis of variance, and the differences between two groups were tested using an unpaired t test; P ≤ .05 was considered significant. Data are presented as mean ± SEM (n = 30 in each group). *p ≤ 0.05 between groups.
Mentions: Differential cell counts were performed on cytospins obtained from BAL fluid. In BAL, the most abundant cells observed in the ARDS, TB and TB + ARDS groups were neutrophils (Figure 3A) which comprised 67.8 ± 5.8%, 78.2 ± 3.0 and 91 ± 4.4% of the total cells in each sample, respectively (Figure 3B). The numbers of BAL neutrophils were significantly greater between TB alone and ARDS alone groups (P < 0.05) and between the TB alone and ARDS + TB groups.

Bottom Line: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups.In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB.This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Tuberculosis (TB) is a rare but known cause of acute respiratory distress syndrome (ARDS). The role of inflammatory cytokines in the progression of ARDS in TB patients is unknown.

Objectives: In this study we investigated the possible link between the levels of inflammatory cytokines in bronchoalveolar lavage (BAL) in patients with TB or ARDS alone or in patients with TB-induced ARDS (ARDS + TB).

Methods: 90 patients were studied: 30 with TB alone, 30 with ARDS alone and 30 with ARDS + TB. BAL was collected by fiberoptic bronchoscopy and the concentrations of interleukin(IL)-6, CXCL8, TNF-α and IL-1β and the amounts of total protein were measured by ELISA and bicinchoninic acid assay (BCA) methods respectively. The correlation between disease severity measured by Murray scores, SOFA and APACHE II analysis and BAL mediators and cells was also determined.

Results: CXCL8 levels in BAL were significantly higher in the ARDS + TB group compared to TB and ARDS alone groups. Disease severity in the ARDS + TB group as determined by Murray score correlated with BAL CXCL8 and neutrophils but not with IL-6, IL-1β and TNF-α concentrations. In addition, CXCL8 levels and neutrophils were increased in non-miliary TB versus miliary TB. This difference in CXCL8 was lost in the presence of ARDS.

Conclusions: BAL CXCL8 levels were significantly higher in patients with ARDS induced by TB and could suggest an important role of CXCL8 in the pathogenesis of this form of ARDS. This further suggests that CXCL8 inhibitors or blockers may be useful to control the onset and/or development of these combined diseases.

No MeSH data available.


Related in: MedlinePlus