Limits...
Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

Show MeSH
Effect of LM1 and LM2 on DNA fragment in CAL-27. CAL-27 cells were treated withLM1 and LM2 for different time periods and then collected. DNA was separated,followed by DNA gel electrophoresis (M, Marker: 2,000 bp, 1,000 bp, 750 bp, 250bp, 100 bp)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4126823&req=5

f04: Effect of LM1 and LM2 on DNA fragment in CAL-27. CAL-27 cells were treated withLM1 and LM2 for different time periods and then collected. DNA was separated,followed by DNA gel electrophoresis (M, Marker: 2,000 bp, 1,000 bp, 750 bp, 250bp, 100 bp)

Mentions: By using agarose gel electrophoresis, significant DNA ladders were detected afterCAL-27 cells were exposed to 36 mg/mL of LM1 and LM2 for a different time period(Figure 4). These results confirmed that LM1and LM2 were able to induce late apoptosis in CAL-27 cells.


Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Effect of LM1 and LM2 on DNA fragment in CAL-27. CAL-27 cells were treated withLM1 and LM2 for different time periods and then collected. DNA was separated,followed by DNA gel electrophoresis (M, Marker: 2,000 bp, 1,000 bp, 750 bp, 250bp, 100 bp)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126823&req=5

f04: Effect of LM1 and LM2 on DNA fragment in CAL-27. CAL-27 cells were treated withLM1 and LM2 for different time periods and then collected. DNA was separated,followed by DNA gel electrophoresis (M, Marker: 2,000 bp, 1,000 bp, 750 bp, 250bp, 100 bp)
Mentions: By using agarose gel electrophoresis, significant DNA ladders were detected afterCAL-27 cells were exposed to 36 mg/mL of LM1 and LM2 for a different time period(Figure 4). These results confirmed that LM1and LM2 were able to induce late apoptosis in CAL-27 cells.

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

Show MeSH