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Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

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Effect of LM1 and LM2 on apoptosis of CAL-27 cells. A: blank control group; B:CAL-27 cells were treated with LM1 for 10 h; C: CAL-27 cells were treated withLM2 for 12 h. After staining with annexin V and PI, the cells were subjected toflow cytometry analysis
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f03: Effect of LM1 and LM2 on apoptosis of CAL-27 cells. A: blank control group; B:CAL-27 cells were treated with LM1 for 10 h; C: CAL-27 cells were treated withLM2 for 12 h. After staining with annexin V and PI, the cells were subjected toflow cytometry analysis

Mentions: To investigate the apoptosis-inducing effects of LM1 and LM2, CAL-27 cells treatedwith a concentration of 36 mg/mL LM1 for 10 h and LM2 for 12 h were analyzed byannexin V and PI staining on a flow cytometer. The induction of apoptosis in CAL-27cells was determined by a flow cytometer (Figure3). For LM1, the percentages of early apoptotic CAL-27 cells was 12.8%,while the percentages of the late cells was 47.3%; for LM2, the percentages of earlyapoptotic CAL-27 cells was 11.22%, while the percentages of the late cells was69.48%.


Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Effect of LM1 and LM2 on apoptosis of CAL-27 cells. A: blank control group; B:CAL-27 cells were treated with LM1 for 10 h; C: CAL-27 cells were treated withLM2 for 12 h. After staining with annexin V and PI, the cells were subjected toflow cytometry analysis
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126823&req=5

f03: Effect of LM1 and LM2 on apoptosis of CAL-27 cells. A: blank control group; B:CAL-27 cells were treated with LM1 for 10 h; C: CAL-27 cells were treated withLM2 for 12 h. After staining with annexin V and PI, the cells were subjected toflow cytometry analysis
Mentions: To investigate the apoptosis-inducing effects of LM1 and LM2, CAL-27 cells treatedwith a concentration of 36 mg/mL LM1 for 10 h and LM2 for 12 h were analyzed byannexin V and PI staining on a flow cytometer. The induction of apoptosis in CAL-27cells was determined by a flow cytometer (Figure3). For LM1, the percentages of early apoptotic CAL-27 cells was 12.8%,while the percentages of the late cells was 47.3%; for LM2, the percentages of earlyapoptotic CAL-27 cells was 11.22%, while the percentages of the late cells was69.48%.

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

Show MeSH