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Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

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Morphological change of CAL 27 cells with apoptotic features. CAL-27 cells weretreated with LM1 and LM2 for 16 h. The cells were stained by Ao and thenphotographed using a fluorescent microscope (x200). The arrow indicatesapoptotic cells
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f02: Morphological change of CAL 27 cells with apoptotic features. CAL-27 cells weretreated with LM1 and LM2 for 16 h. The cells were stained by Ao and thenphotographed using a fluorescent microscope (x200). The arrow indicatesapoptotic cells

Mentions: To evaluate whether the cytotoxic effects induced by LM1 and LM2 were caused byapoptosis, nuclear morphology was observed using Acridine orange staining underfluorescence microscope. After being treated with LM1 and LM2 at a 36 mg/mLconcentration for 16 h, cell morphology became abnormal with cell nuclear shrinkage,and chromatin condensation was induced to occur in the treated CAL-27 cells (Figure 2). It was suggested that the number ofapoptotic cells significantly increased in comparison with control.


Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Morphological change of CAL 27 cells with apoptotic features. CAL-27 cells weretreated with LM1 and LM2 for 16 h. The cells were stained by Ao and thenphotographed using a fluorescent microscope (x200). The arrow indicatesapoptotic cells
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126823&req=5

f02: Morphological change of CAL 27 cells with apoptotic features. CAL-27 cells weretreated with LM1 and LM2 for 16 h. The cells were stained by Ao and thenphotographed using a fluorescent microscope (x200). The arrow indicatesapoptotic cells
Mentions: To evaluate whether the cytotoxic effects induced by LM1 and LM2 were caused byapoptosis, nuclear morphology was observed using Acridine orange staining underfluorescence microscope. After being treated with LM1 and LM2 at a 36 mg/mLconcentration for 16 h, cell morphology became abnormal with cell nuclear shrinkage,and chromatin condensation was induced to occur in the treated CAL-27 cells (Figure 2). It was suggested that the number ofapoptotic cells significantly increased in comparison with control.

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

Show MeSH