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Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

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Effect of LM1 and LM2 on viability of CAL-27 cells. Cells were cultured inDMEM+10% FBS with different concentrations of LM1 and LM2 for 72 h. Inhibitionrates were determined as described in Material and Methods. Each point is mean± Standard Deviation of three experiments.*p<0.01 compared to control (0mg/mL)
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f01: Effect of LM1 and LM2 on viability of CAL-27 cells. Cells were cultured inDMEM+10% FBS with different concentrations of LM1 and LM2 for 72 h. Inhibitionrates were determined as described in Material and Methods. Each point is mean± Standard Deviation of three experiments.*p<0.01 compared to control (0mg/mL)

Mentions: CAL-27 cells were used for investigating the effect of LM1 and LM2 on the viabilityof human tongue squamous cells CAL-27 by MTT assay in vitro. It wasshown that 3-48 mg/mL LM1 or LM2 inhibited cell growth in a dose-dependent manner(Figure 1). The half maximal inhibitoryconcentration (IC50) for LM1 and LM2 was found to be 15.1 mg/mL and 10.27mg/mL respectively. It was shown that LM1 and LM2 were able to induce cytotoxicity inCAL-27 cells. Nevertheless, no statistically significant difference in cytotoxicityto CAL-27 was found between LM1 and LM2 (P>0.05).


Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.

Zhang G, Zhang J, Wang X, Yang W, Sun Z, Kumar CN, Guan H, Guan J - J Appl Oral Sci (2014 Jul-Aug)

Effect of LM1 and LM2 on viability of CAL-27 cells. Cells were cultured inDMEM+10% FBS with different concentrations of LM1 and LM2 for 72 h. Inhibitionrates were determined as described in Material and Methods. Each point is mean± Standard Deviation of three experiments.*p<0.01 compared to control (0mg/mL)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126823&req=5

f01: Effect of LM1 and LM2 on viability of CAL-27 cells. Cells were cultured inDMEM+10% FBS with different concentrations of LM1 and LM2 for 72 h. Inhibitionrates were determined as described in Material and Methods. Each point is mean± Standard Deviation of three experiments.*p<0.01 compared to control (0mg/mL)
Mentions: CAL-27 cells were used for investigating the effect of LM1 and LM2 on the viabilityof human tongue squamous cells CAL-27 by MTT assay in vitro. It wasshown that 3-48 mg/mL LM1 or LM2 inhibited cell growth in a dose-dependent manner(Figure 1). The half maximal inhibitoryconcentration (IC50) for LM1 and LM2 was found to be 15.1 mg/mL and 10.27mg/mL respectively. It was shown that LM1 and LM2 were able to induce cytotoxicity inCAL-27 cells. Nevertheless, no statistically significant difference in cytotoxicityto CAL-27 was found between LM1 and LM2 (P>0.05).

Bottom Line: To study the effect of Lactobacillus sp.A-2 metabolites; Lactobacillus sp.A-2 metabolites and their molecular mechanism is in progress.

View Article: PubMed Central - PubMed

Affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.

ABSTRACT

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.

Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.

Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.

Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

Show MeSH