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Sensing Reversible Protein-Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors.

Münzer AM, Seo W, Morgan GJ, Michael ZP, Zhao Y, Melzer K, Scarpa G, Star A - J Phys Chem C Nanomater Interfaces (2014)

Bottom Line: We report on the reversible detection of CaptAvidin, a tyrosine modified avidin, with single-walled carbon nanotube (SWNT) field-effect transistors (FETs) noncovalently functionalized with biotin moieties using 1-pyrenebutyric acid as a linker.Binding affinities at different pH values were quantified, and the sensor's response at various ionic strengths was analyzed.Moreover, gold nanoparticle decorated SWNT FETs were functionalized with biotin using 1-pyrenebutyric acid as a linker for the CNT surface and (±)-α-lipoic acid linkers for the gold surface, and reversible CaptAvidin binding is shown, paving the way for potential dual mode measurements with the addition of surface enhanced Raman spectroscopy (SERS).

View Article: PubMed Central - PubMed

Affiliation: Institute for Nanoelectronics, Technische Universität München , Arcisstraße 21, 80333, Munich, Germany.

ABSTRACT
We report on the reversible detection of CaptAvidin, a tyrosine modified avidin, with single-walled carbon nanotube (SWNT) field-effect transistors (FETs) noncovalently functionalized with biotin moieties using 1-pyrenebutyric acid as a linker. Binding affinities at different pH values were quantified, and the sensor's response at various ionic strengths was analyzed. Furthermore, protein "fingerprints" of NeutrAvidin and streptavidin were obtained by monitoring their adsorption at several pH values. Moreover, gold nanoparticle decorated SWNT FETs were functionalized with biotin using 1-pyrenebutyric acid as a linker for the CNT surface and (±)-α-lipoic acid linkers for the gold surface, and reversible CaptAvidin binding is shown, paving the way for potential dual mode measurements with the addition of surface enhanced Raman spectroscopy (SERS).

No MeSH data available.


Related in: MedlinePlus

(a) SEM imageof gold nanoparticle decorated SWNTs. The particlesizes obtained from the applied parameters for this device are 50to 200 nm. (b) Raman spectra of gold nanoparticle decorated SWNTsusing varying deposition times of bulk electrolysis (deposition voltage−0.4 V). (c) Transfer characteristics of a SWNT FET recordedbefore and after functionalization with gold nanoparticles. (d) Transfercharacteristics recorded for CaptAvidin detection with a LA-B functionalizeddevice. (1) Transfer curve was taken before exposure to CaptAvidin,(2) after incubation with 140 nM CaptAvidin, and (3) after 15 minexposure to pH 10 buffer. (e) Raman spectra of gold nanoparticle-decoratedSWNTs functionalized with LA-B after incubation with CaptAvidin andafter pH 10 washing. Peaks unique to the protein can be discernedafter incubation which disappear upon rinsing.
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fig3: (a) SEM imageof gold nanoparticle decorated SWNTs. The particlesizes obtained from the applied parameters for this device are 50to 200 nm. (b) Raman spectra of gold nanoparticle decorated SWNTsusing varying deposition times of bulk electrolysis (deposition voltage−0.4 V). (c) Transfer characteristics of a SWNT FET recordedbefore and after functionalization with gold nanoparticles. (d) Transfercharacteristics recorded for CaptAvidin detection with a LA-B functionalizeddevice. (1) Transfer curve was taken before exposure to CaptAvidin,(2) after incubation with 140 nM CaptAvidin, and (3) after 15 minexposure to pH 10 buffer. (e) Raman spectra of gold nanoparticle-decoratedSWNTs functionalized with LA-B after incubation with CaptAvidin andafter pH 10 washing. Peaks unique to the protein can be discernedafter incubation which disappear upon rinsing.

Mentions: There is an increasinginterest in surface enhanced Raman spectroscopy(SERS) for applications in chemical and biological sensing, due toits unique sensitivity with regards to molecular fingerprinting.31−33 Surface enhanced Raman spectroscopy can be realized by decoratingcarbon nanotubes with gold nanoparticles directly on chip by electrodepositionfrom a AuCl3 solution.34,35 To assessthe feasibility of this approach for solution processed SWNT FETs,we utilized AuNP-SWNT hybrid devices, the fabrication of which isdetailed in the Experimental Section. In Figure 3a, a scanning electron microscope image of a golddecorated device (deposition parameters −0.4 V for 10 s) revealsa distribution of particle sizes ranging from 50 to 200 nm.


Sensing Reversible Protein-Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors.

Münzer AM, Seo W, Morgan GJ, Michael ZP, Zhao Y, Melzer K, Scarpa G, Star A - J Phys Chem C Nanomater Interfaces (2014)

(a) SEM imageof gold nanoparticle decorated SWNTs. The particlesizes obtained from the applied parameters for this device are 50to 200 nm. (b) Raman spectra of gold nanoparticle decorated SWNTsusing varying deposition times of bulk electrolysis (deposition voltage−0.4 V). (c) Transfer characteristics of a SWNT FET recordedbefore and after functionalization with gold nanoparticles. (d) Transfercharacteristics recorded for CaptAvidin detection with a LA-B functionalizeddevice. (1) Transfer curve was taken before exposure to CaptAvidin,(2) after incubation with 140 nM CaptAvidin, and (3) after 15 minexposure to pH 10 buffer. (e) Raman spectra of gold nanoparticle-decoratedSWNTs functionalized with LA-B after incubation with CaptAvidin andafter pH 10 washing. Peaks unique to the protein can be discernedafter incubation which disappear upon rinsing.
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Related In: Results  -  Collection

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fig3: (a) SEM imageof gold nanoparticle decorated SWNTs. The particlesizes obtained from the applied parameters for this device are 50to 200 nm. (b) Raman spectra of gold nanoparticle decorated SWNTsusing varying deposition times of bulk electrolysis (deposition voltage−0.4 V). (c) Transfer characteristics of a SWNT FET recordedbefore and after functionalization with gold nanoparticles. (d) Transfercharacteristics recorded for CaptAvidin detection with a LA-B functionalizeddevice. (1) Transfer curve was taken before exposure to CaptAvidin,(2) after incubation with 140 nM CaptAvidin, and (3) after 15 minexposure to pH 10 buffer. (e) Raman spectra of gold nanoparticle-decoratedSWNTs functionalized with LA-B after incubation with CaptAvidin andafter pH 10 washing. Peaks unique to the protein can be discernedafter incubation which disappear upon rinsing.
Mentions: There is an increasinginterest in surface enhanced Raman spectroscopy(SERS) for applications in chemical and biological sensing, due toits unique sensitivity with regards to molecular fingerprinting.31−33 Surface enhanced Raman spectroscopy can be realized by decoratingcarbon nanotubes with gold nanoparticles directly on chip by electrodepositionfrom a AuCl3 solution.34,35 To assessthe feasibility of this approach for solution processed SWNT FETs,we utilized AuNP-SWNT hybrid devices, the fabrication of which isdetailed in the Experimental Section. In Figure 3a, a scanning electron microscope image of a golddecorated device (deposition parameters −0.4 V for 10 s) revealsa distribution of particle sizes ranging from 50 to 200 nm.

Bottom Line: We report on the reversible detection of CaptAvidin, a tyrosine modified avidin, with single-walled carbon nanotube (SWNT) field-effect transistors (FETs) noncovalently functionalized with biotin moieties using 1-pyrenebutyric acid as a linker.Binding affinities at different pH values were quantified, and the sensor's response at various ionic strengths was analyzed.Moreover, gold nanoparticle decorated SWNT FETs were functionalized with biotin using 1-pyrenebutyric acid as a linker for the CNT surface and (±)-α-lipoic acid linkers for the gold surface, and reversible CaptAvidin binding is shown, paving the way for potential dual mode measurements with the addition of surface enhanced Raman spectroscopy (SERS).

View Article: PubMed Central - PubMed

Affiliation: Institute for Nanoelectronics, Technische Universität München , Arcisstraße 21, 80333, Munich, Germany.

ABSTRACT
We report on the reversible detection of CaptAvidin, a tyrosine modified avidin, with single-walled carbon nanotube (SWNT) field-effect transistors (FETs) noncovalently functionalized with biotin moieties using 1-pyrenebutyric acid as a linker. Binding affinities at different pH values were quantified, and the sensor's response at various ionic strengths was analyzed. Furthermore, protein "fingerprints" of NeutrAvidin and streptavidin were obtained by monitoring their adsorption at several pH values. Moreover, gold nanoparticle decorated SWNT FETs were functionalized with biotin using 1-pyrenebutyric acid as a linker for the CNT surface and (±)-α-lipoic acid linkers for the gold surface, and reversible CaptAvidin binding is shown, paving the way for potential dual mode measurements with the addition of surface enhanced Raman spectroscopy (SERS).

No MeSH data available.


Related in: MedlinePlus