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Biological Targeting of Plasmonic Nanoparticles Improves Cellular Imaging via the Enhanced Scattering in the Aggregates Formed.

Aioub M, Kang B, Mackey MA, El-Sayed MA - J Phys Chem Lett (2014)

Bottom Line: Nuclear-targeted AuNPs showed the greatest scattering due to the formation of denser nanoparticle clusters (i.e., increased localization).We also obtained a dynamic profile of AuNP localization in living cells, indicating that nuclear localization is directly related to the number of nuclear-targeting peptides on the AuNP surface.Increased localization led to increased plasmonic field coupling, resulting in significantly higher scattering intensity.

View Article: PubMed Central - PubMed

Affiliation: Laser Dynamics Laboratory, School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

ABSTRACT
Gold nanoparticles (AuNPs) demonstrate great promise in biomedical applications due to their plasmonically enhanced imaging properties. When in close proximity, AuNPs plasmonic fields couple together, increasing their scattering cross-section due to the formation of hot spots, improving their imaging utility. In the present study, we modified the AuNPs surface with different peptides to target the nucleus and/or the cell as a whole, resulting in similar cellular uptake but different scattering intensities. Nuclear-targeted AuNPs showed the greatest scattering due to the formation of denser nanoparticle clusters (i.e., increased localization). We also obtained a dynamic profile of AuNP localization in living cells, indicating that nuclear localization is directly related to the number of nuclear-targeting peptides on the AuNP surface. Increased localization led to increased plasmonic field coupling, resulting in significantly higher scattering intensity. Thus, biochemical targeting of plasmonic nanoparticles to subcellular components is expected to lead to more resolved imaging of cellular processes.

No MeSH data available.


Related in: MedlinePlus

Real-timeRayleigh scattering dark-field images of cellular uptakeof gold nanoparticles with different surface modifications: (A) RGD-AuNPs,(B) RGD1/NLS1-AuNPs, (C) RGD1/NLS10-AuNPs. Scale bar: 10 μm.
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fig1: Real-timeRayleigh scattering dark-field images of cellular uptakeof gold nanoparticles with different surface modifications: (A) RGD-AuNPs,(B) RGD1/NLS1-AuNPs, (C) RGD1/NLS10-AuNPs. Scale bar: 10 μm.

Mentions: Localization Kinetics Using Plasmonically-EnhancedRayleighScattering. To ensure that changes in the observed scatteringintensity were due to AuNP uptake (and not influenced by cell death),a treatment concentration of 0.4 nM AuNP in culture media was chosenfor these studies. After 24 h, the viability of treated cells wasmeasured using an XTT cell viability assay, and no significant celldeath was observed from the three AuNP designs studied (Figure S3). Due to the αβ integrintargeting ability of RGD, all three AuNP samples studied also demonstratedsimilar levels of overall uptake (∼30%, Figure S4). However, the intracellular localization of thedifferent AuNPs varied based on the AuNP surface modification. Assuggested by the live-cell Rayleigh-scattering dark field images inFigure 1, the AuNPs containing NLS peptide(Figure 1B,C) show higher AuNP localizationat the nucleus compared to AuNPs without NLS (Figure 1A). Additionally, an increase in NLS peptide content (RGD1/NLS10-AuNPs) results in a more rapid localizationrate of AuNPs at the nucleus (Figure 1C).


Biological Targeting of Plasmonic Nanoparticles Improves Cellular Imaging via the Enhanced Scattering in the Aggregates Formed.

Aioub M, Kang B, Mackey MA, El-Sayed MA - J Phys Chem Lett (2014)

Real-timeRayleigh scattering dark-field images of cellular uptakeof gold nanoparticles with different surface modifications: (A) RGD-AuNPs,(B) RGD1/NLS1-AuNPs, (C) RGD1/NLS10-AuNPs. Scale bar: 10 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126704&req=5

fig1: Real-timeRayleigh scattering dark-field images of cellular uptakeof gold nanoparticles with different surface modifications: (A) RGD-AuNPs,(B) RGD1/NLS1-AuNPs, (C) RGD1/NLS10-AuNPs. Scale bar: 10 μm.
Mentions: Localization Kinetics Using Plasmonically-EnhancedRayleighScattering. To ensure that changes in the observed scatteringintensity were due to AuNP uptake (and not influenced by cell death),a treatment concentration of 0.4 nM AuNP in culture media was chosenfor these studies. After 24 h, the viability of treated cells wasmeasured using an XTT cell viability assay, and no significant celldeath was observed from the three AuNP designs studied (Figure S3). Due to the αβ integrintargeting ability of RGD, all three AuNP samples studied also demonstratedsimilar levels of overall uptake (∼30%, Figure S4). However, the intracellular localization of thedifferent AuNPs varied based on the AuNP surface modification. Assuggested by the live-cell Rayleigh-scattering dark field images inFigure 1, the AuNPs containing NLS peptide(Figure 1B,C) show higher AuNP localizationat the nucleus compared to AuNPs without NLS (Figure 1A). Additionally, an increase in NLS peptide content (RGD1/NLS10-AuNPs) results in a more rapid localizationrate of AuNPs at the nucleus (Figure 1C).

Bottom Line: Nuclear-targeted AuNPs showed the greatest scattering due to the formation of denser nanoparticle clusters (i.e., increased localization).We also obtained a dynamic profile of AuNP localization in living cells, indicating that nuclear localization is directly related to the number of nuclear-targeting peptides on the AuNP surface.Increased localization led to increased plasmonic field coupling, resulting in significantly higher scattering intensity.

View Article: PubMed Central - PubMed

Affiliation: Laser Dynamics Laboratory, School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

ABSTRACT
Gold nanoparticles (AuNPs) demonstrate great promise in biomedical applications due to their plasmonically enhanced imaging properties. When in close proximity, AuNPs plasmonic fields couple together, increasing their scattering cross-section due to the formation of hot spots, improving their imaging utility. In the present study, we modified the AuNPs surface with different peptides to target the nucleus and/or the cell as a whole, resulting in similar cellular uptake but different scattering intensities. Nuclear-targeted AuNPs showed the greatest scattering due to the formation of denser nanoparticle clusters (i.e., increased localization). We also obtained a dynamic profile of AuNP localization in living cells, indicating that nuclear localization is directly related to the number of nuclear-targeting peptides on the AuNP surface. Increased localization led to increased plasmonic field coupling, resulting in significantly higher scattering intensity. Thus, biochemical targeting of plasmonic nanoparticles to subcellular components is expected to lead to more resolved imaging of cellular processes.

No MeSH data available.


Related in: MedlinePlus