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Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

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Shear flow testing via a microfluidic based testing platform.(A) Shear stress of 1 dyn/cm2 was applied for 12 min; (B) Diagram represents shear stress level applied, 2 min at 0.25 dyn/cm2, 2 min at 0.5 dyn/cm2, 2 min at 1 dyn/cm2, 2 min at 2.5 dyn/cm2, 2 min at 5 dyn/cm2, 2 min at 10 dyn/cm2. Data presented as mean ± SEM (n = 3).
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pone-0104502-g008: Shear flow testing via a microfluidic based testing platform.(A) Shear stress of 1 dyn/cm2 was applied for 12 min; (B) Diagram represents shear stress level applied, 2 min at 0.25 dyn/cm2, 2 min at 0.5 dyn/cm2, 2 min at 1 dyn/cm2, 2 min at 2.5 dyn/cm2, 2 min at 5 dyn/cm2, 2 min at 10 dyn/cm2. Data presented as mean ± SEM (n = 3).

Mentions: To measure the cell adhesion strength, five control experiments were performed to examine cells detachment over time and as a function of shear stress. The flow was applied in the direction of both perpendicular and parallel to the grooves and waves. In the first experiment, the shear flow was fixed at 1 dyn/cm2 for 12 min. This particular shear level corresponds to the lower end of physiological shear stress in the blood vessels which ranges from 1 dyn/cm2 to 70 dyn/cm2[45]. The number of attached cells decreased quickly within the first 2 min, and plateaued afterwards, as shown in Fig. 8A. This suggested that at a given shear level cells detached quickly rather than gradually peeled off over time. Thus, two minutes is enough to establish a steady number of cell detachment.


Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Shear flow testing via a microfluidic based testing platform.(A) Shear stress of 1 dyn/cm2 was applied for 12 min; (B) Diagram represents shear stress level applied, 2 min at 0.25 dyn/cm2, 2 min at 0.5 dyn/cm2, 2 min at 1 dyn/cm2, 2 min at 2.5 dyn/cm2, 2 min at 5 dyn/cm2, 2 min at 10 dyn/cm2. Data presented as mean ± SEM (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126693&req=5

pone-0104502-g008: Shear flow testing via a microfluidic based testing platform.(A) Shear stress of 1 dyn/cm2 was applied for 12 min; (B) Diagram represents shear stress level applied, 2 min at 0.25 dyn/cm2, 2 min at 0.5 dyn/cm2, 2 min at 1 dyn/cm2, 2 min at 2.5 dyn/cm2, 2 min at 5 dyn/cm2, 2 min at 10 dyn/cm2. Data presented as mean ± SEM (n = 3).
Mentions: To measure the cell adhesion strength, five control experiments were performed to examine cells detachment over time and as a function of shear stress. The flow was applied in the direction of both perpendicular and parallel to the grooves and waves. In the first experiment, the shear flow was fixed at 1 dyn/cm2 for 12 min. This particular shear level corresponds to the lower end of physiological shear stress in the blood vessels which ranges from 1 dyn/cm2 to 70 dyn/cm2[45]. The number of attached cells decreased quickly within the first 2 min, and plateaued afterwards, as shown in Fig. 8A. This suggested that at a given shear level cells detached quickly rather than gradually peeled off over time. Thus, two minutes is enough to establish a steady number of cell detachment.

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

Show MeSH
Related in: MedlinePlus