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Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

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Alignment of BAOECs on 20 µm micro-wavy substrates after 24 h incubation.(A–C) Histograms of cell number, alignment angle, and cell location on 20 µm wavy surfaces (n = 100). Error bars, SEM.
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pone-0104502-g006: Alignment of BAOECs on 20 µm micro-wavy substrates after 24 h incubation.(A–C) Histograms of cell number, alignment angle, and cell location on 20 µm wavy surfaces (n = 100). Error bars, SEM.

Mentions: The cell orientation histograms are presented in Fig. 6 A–C. Fig. 6A shows that most cells were located in the troughs of the wave. Fig. 6B shows that about 60% of the cells had a <10° alignment angle, with a significant fraction of them very close to 0° on the 20 µm wavy surface. The spread endothelial cells show strong alignment within the wavy pattern. The alignment angle and wave location of each cell are shown in Fig. 6C. A wider angle distribution of cells is observed at troughs of the wave, while a narrow angle distribution of cells is observed at the crests of the wave.


Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Alignment of BAOECs on 20 µm micro-wavy substrates after 24 h incubation.(A–C) Histograms of cell number, alignment angle, and cell location on 20 µm wavy surfaces (n = 100). Error bars, SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126693&req=5

pone-0104502-g006: Alignment of BAOECs on 20 µm micro-wavy substrates after 24 h incubation.(A–C) Histograms of cell number, alignment angle, and cell location on 20 µm wavy surfaces (n = 100). Error bars, SEM.
Mentions: The cell orientation histograms are presented in Fig. 6 A–C. Fig. 6A shows that most cells were located in the troughs of the wave. Fig. 6B shows that about 60% of the cells had a <10° alignment angle, with a significant fraction of them very close to 0° on the 20 µm wavy surface. The spread endothelial cells show strong alignment within the wavy pattern. The alignment angle and wave location of each cell are shown in Fig. 6C. A wider angle distribution of cells is observed at troughs of the wave, while a narrow angle distribution of cells is observed at the crests of the wave.

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

Show MeSH
Related in: MedlinePlus