Limits...
Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

Show MeSH

Related in: MedlinePlus

Images of BAOECs on different substrates.(A) Microscope image of cells on flat PDMS substrate after 24 h; (B) Microscope image of cells on 20 µm spacing, 6.6 µm height micro-wave after 24 h; (C) Microscope image of cells on the micro-wave after 48 h; (D) SEM image of a BAOEC on micro-wavy substrate after 24 h. In Fig. D, the cell looks smaller than in Fig. A–C because of the dehydration process in preparation for SEM.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4126693&req=5

pone-0104502-g005: Images of BAOECs on different substrates.(A) Microscope image of cells on flat PDMS substrate after 24 h; (B) Microscope image of cells on 20 µm spacing, 6.6 µm height micro-wave after 24 h; (C) Microscope image of cells on the micro-wave after 48 h; (D) SEM image of a BAOEC on micro-wavy substrate after 24 h. In Fig. D, the cell looks smaller than in Fig. A–C because of the dehydration process in preparation for SEM.

Mentions: After 24 h of cell culturing, while BAOECs on the flat surface were randomly oriented (Fig. 5A), those on the micro-wavy surface were found oriented along the long axis of the waves (Fig. 5B). The BAOECs on the flat surface also appeared rounder than those on the wavy surface. Comparing the images of the wavy surface captured after 24 h and 48 h (Fig. 5B and 5C), no significant differences were observed on the distribution or spreading of the cells; thus, the incubation time of 24 h was utilized for further analysis. In addition, scanning electron microscopy (SEM) images revealed that the cell aligned along the wave and was located in the trough of the wave (Fig. 5D). In preparation for SEM, cells were fixed in 4% paraformaldehyde for 1 h and washed with PBS; then, the cells were dehydrated in 20%, 30%, 50%, 70%, 85%, 95%, and 100% (volume/volume) ethanol concentration gradient solutions (each for 15 min), followed by 24 h dry out in the refrigerator. Thus, in the SEM image (Fig. 5D), cells look smaller than in the optical images (Fig. 5 A–C).


Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Images of BAOECs on different substrates.(A) Microscope image of cells on flat PDMS substrate after 24 h; (B) Microscope image of cells on 20 µm spacing, 6.6 µm height micro-wave after 24 h; (C) Microscope image of cells on the micro-wave after 48 h; (D) SEM image of a BAOEC on micro-wavy substrate after 24 h. In Fig. D, the cell looks smaller than in Fig. A–C because of the dehydration process in preparation for SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126693&req=5

pone-0104502-g005: Images of BAOECs on different substrates.(A) Microscope image of cells on flat PDMS substrate after 24 h; (B) Microscope image of cells on 20 µm spacing, 6.6 µm height micro-wave after 24 h; (C) Microscope image of cells on the micro-wave after 48 h; (D) SEM image of a BAOEC on micro-wavy substrate after 24 h. In Fig. D, the cell looks smaller than in Fig. A–C because of the dehydration process in preparation for SEM.
Mentions: After 24 h of cell culturing, while BAOECs on the flat surface were randomly oriented (Fig. 5A), those on the micro-wavy surface were found oriented along the long axis of the waves (Fig. 5B). The BAOECs on the flat surface also appeared rounder than those on the wavy surface. Comparing the images of the wavy surface captured after 24 h and 48 h (Fig. 5B and 5C), no significant differences were observed on the distribution or spreading of the cells; thus, the incubation time of 24 h was utilized for further analysis. In addition, scanning electron microscopy (SEM) images revealed that the cell aligned along the wave and was located in the trough of the wave (Fig. 5D). In preparation for SEM, cells were fixed in 4% paraformaldehyde for 1 h and washed with PBS; then, the cells were dehydrated in 20%, 30%, 50%, 70%, 85%, 95%, and 100% (volume/volume) ethanol concentration gradient solutions (each for 15 min), followed by 24 h dry out in the refrigerator. Thus, in the SEM image (Fig. 5D), cells look smaller than in the optical images (Fig. 5 A–C).

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

Show MeSH
Related in: MedlinePlus