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Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

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Cell distribution at the initial seeding.(A) Microscope image of cells on a wavy surface with 20 µm spacing and 6.6 µm height; (B) The number of endothelial cells at different wave locations.
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pone-0104502-g004: Cell distribution at the initial seeding.(A) Microscope image of cells on a wavy surface with 20 µm spacing and 6.6 µm height; (B) The number of endothelial cells at different wave locations.

Mentions: BAOECs were seeded onto the wavy surface with a wavelength of 20 µm. At the initial seeding, the rounded BAOECs were almost uniformly distributed on the wavy surface (Fig. 4A). To quantify the cell distribution, the 20 µm wavelength was divided into 1 µm segment locations and a histogram of the number of cells in each segment was created (Fig. 4B). In the phase-contrast microscope image, the white lines represent the crest of the micro-wavy surface. Most cells tend to be located at the troughs of the wavy surface, which demonstrates that wavy surfaces have good control of cell position.


Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Cell distribution at the initial seeding.(A) Microscope image of cells on a wavy surface with 20 µm spacing and 6.6 µm height; (B) The number of endothelial cells at different wave locations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126693&req=5

pone-0104502-g004: Cell distribution at the initial seeding.(A) Microscope image of cells on a wavy surface with 20 µm spacing and 6.6 µm height; (B) The number of endothelial cells at different wave locations.
Mentions: BAOECs were seeded onto the wavy surface with a wavelength of 20 µm. At the initial seeding, the rounded BAOECs were almost uniformly distributed on the wavy surface (Fig. 4A). To quantify the cell distribution, the 20 µm wavelength was divided into 1 µm segment locations and a histogram of the number of cells in each segment was created (Fig. 4B). In the phase-contrast microscope image, the white lines represent the crest of the micro-wavy surface. Most cells tend to be located at the troughs of the wavy surface, which demonstrates that wavy surfaces have good control of cell position.

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

Show MeSH
Related in: MedlinePlus