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Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

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Illustration of the fabrication process of microwavy patterns.(A–D) Uniaxial stretching of PDMS films at various mechanical stretch settings to generate microwavy patterns.
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pone-0104502-g002: Illustration of the fabrication process of microwavy patterns.(A–D) Uniaxial stretching of PDMS films at various mechanical stretch settings to generate microwavy patterns.

Mentions: Microgrooved surfaces used in cell capture or particle isolation can be fabricated through standard photolithography, followed by PDMS molding techniques [24]. However, these surfaces typically have sharp corners, which are not favorable for cell seeding, spreading, and adhesion [28], [30]. To fabricate a smooth microwavy surface, we used bucking of oxide/PDMS bilayer, as shown in Fig. 2.


Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.

Hu J, Hardy C, Chen CM, Yang S, Voloshin AS, Liu Y - PLoS ONE (2014)

Illustration of the fabrication process of microwavy patterns.(A–D) Uniaxial stretching of PDMS films at various mechanical stretch settings to generate microwavy patterns.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126693&req=5

pone-0104502-g002: Illustration of the fabrication process of microwavy patterns.(A–D) Uniaxial stretching of PDMS films at various mechanical stretch settings to generate microwavy patterns.
Mentions: Microgrooved surfaces used in cell capture or particle isolation can be fabricated through standard photolithography, followed by PDMS molding techniques [24]. However, these surfaces typically have sharp corners, which are not favorable for cell seeding, spreading, and adhesion [28], [30]. To fabricate a smooth microwavy surface, we used bucking of oxide/PDMS bilayer, as shown in Fig. 2.

Bottom Line: To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h.As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern.The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.

ABSTRACT
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.

Show MeSH
Related in: MedlinePlus