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Identification of the main allergen sensitizers in an Iran asthmatic population by molecular diagnosis.

Teifoori F, Shams-Ghahfarokhi M, Postigo I, Razzaghi-Abyaneh M, Eslamifar A, Gutiérrez A, Suñén E, Martínez J - Allergy Asthma Clin Immunol (2014)

Bottom Line: SDS-PAGE IgE-immunoblotting associated with mass spectrometry was carried out to study the cockroach IgE-binding sensitizing proteins.Pollen and cockroach were the most relevant allergen sources in the asthmatic population.Using the Component Resolved Diagnosis, it was possible to identify a new Blattella germanica cockroach allergen (Blattella alpha amylase 53 kDa) that could sensitize a relevant percentage of this population.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-331, Iran ; Laboratory of Parasitology and Allergy, Center for Research Lascaray Ikergunea, University of the Basque Country, Pº Universidad, 7, 01006 Vitoria, Spain ; Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, Pº Universidad, 7, 01006 Vitoria, Spain.

ABSTRACT

Background: There has been a significant growth in the prevalence of allergy, mainly associated to IgE-mediated disorders such as asthma and rhinitis. The identification of atopy in asthmatic patients through the measurement of specific IgE can help to identify risk factors that cause asthmatic symptoms in patients. The development and use of individualized allergen-based tests by the Component Resolved Diagnosis has been a crucial advance in the accurate diagnosis and control of allergic patients. The objective of this work was to assess the usefulness of molecular diagnosis to identify environmental allergens as possible factors influencing the development and manifestation of asthma in a group of asthmatic patients from Iran.

Studied population: 202 adult asthmatic patients treated at the Loghman Hakim Hospital and Pasteur Institute of Teheran (Iran) from 2011 to 2012. Specific IgE determined by the ImmunoCAP system were used to both evaluate the patients' atopic condition and the molecules involved in the allergic sensitization. SDS-PAGE IgE-immunoblotting associated with mass spectrometry was carried out to study the cockroach IgE-binding sensitizing proteins.

Results: Forty-five percent of all patients could be considered atopic individuals. Eighty-two percent of atopic patients were sensitized to pollen allergens. The Salsola kali (Sal k 1) and the Phleum pratense (rPhl p 1 and/or rPhl p 5) major allergens were the most common sensitizers among pollens (71% and 18%, respectively). Thirty-five percent of the atopic population was sensitized to cockroach. Four different allergens, including a previously unknown alpha-amylase, were identified in the cockroach extract. No significant associations could be demonstrated between the severity of asthma and the specific IgE levels in the atopic population. Statistical analysis identified the Sal k 1 as the main protein allergen influencing the development and expression of asthma in the studied population.

Conclusions: Pollen and cockroach were the most relevant allergen sources in the asthmatic population. The Salsola kali major allergen was the main cause for sensitization in the atopic patients suffering asthma. Using the Component Resolved Diagnosis, it was possible to identify a new Blattella germanica cockroach allergen (Blattella alpha amylase 53 kDa) that could sensitize a relevant percentage of this population.

No MeSH data available.


Related in: MedlinePlus

Allergogram of 22/33 atopic patients sensitized to cockroach crude extracts.
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Figure 1: Allergogram of 22/33 atopic patients sensitized to cockroach crude extracts.

Mentions: To identify which cockroach allergens were able to sensitize the atopic population, SDS-PAGE immunoblotting associated with MALDI-TOF-MS and MALDI-TOF-MS/MS were performed. Figure 1 shows the allergogram of the 22 from 33 atopic patients sensitized to cockroach crude extracts. Four IgE-binding components of 75.0, 53.0, 42.0 and 36.0 kDa molecular weight (MW), reactive in 63.6%, 86.4%, 72.7% and 54.5% of cockroach-sensitized patients, respectively, were revealed in this way. The prevalence rates of each uncovered cockroach allergen (75, 53, 42 and 36 kDa) in the total atopic population with asthma were 15%, 20%, 17% and 12%, respectively. Mass spectrometry analysis of the four bands allowed for the identification of only the 53 and 42 kDa proteins. Table 3 displays the results of mass spectrometry. These two proteins were identified as alpha-amylase and arginine-kinase, which have theoretical MWs of 57.5 and 40.1 kDa, respectively. No specific IgE to Aspergillus oryzae alpha-amylase was found in sera from 8 patients sensitized to Blattella germanica alpha-amylase (≤0.1 kU/L). A BLAST alignment of protein sequences of Blattella germanica alpha-amylase with Aspergillus oryzae alpha amylase showed a query cover and E-value of 50% and 4E-12, respectively. An SDAP search revealed that there was a match of at least of six contiguous amino acids between the alpha-amylase of Blattella germanica and the alpha-amylase of Blomia tropicalis (Blo t 4.0101), Dermatophagoides pteronyssinus (Der p 4) and Euroglyphus maynei (Eur m 4), which are three described mite allergens.


Identification of the main allergen sensitizers in an Iran asthmatic population by molecular diagnosis.

Teifoori F, Shams-Ghahfarokhi M, Postigo I, Razzaghi-Abyaneh M, Eslamifar A, Gutiérrez A, Suñén E, Martínez J - Allergy Asthma Clin Immunol (2014)

Allergogram of 22/33 atopic patients sensitized to cockroach crude extracts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4126640&req=5

Figure 1: Allergogram of 22/33 atopic patients sensitized to cockroach crude extracts.
Mentions: To identify which cockroach allergens were able to sensitize the atopic population, SDS-PAGE immunoblotting associated with MALDI-TOF-MS and MALDI-TOF-MS/MS were performed. Figure 1 shows the allergogram of the 22 from 33 atopic patients sensitized to cockroach crude extracts. Four IgE-binding components of 75.0, 53.0, 42.0 and 36.0 kDa molecular weight (MW), reactive in 63.6%, 86.4%, 72.7% and 54.5% of cockroach-sensitized patients, respectively, were revealed in this way. The prevalence rates of each uncovered cockroach allergen (75, 53, 42 and 36 kDa) in the total atopic population with asthma were 15%, 20%, 17% and 12%, respectively. Mass spectrometry analysis of the four bands allowed for the identification of only the 53 and 42 kDa proteins. Table 3 displays the results of mass spectrometry. These two proteins were identified as alpha-amylase and arginine-kinase, which have theoretical MWs of 57.5 and 40.1 kDa, respectively. No specific IgE to Aspergillus oryzae alpha-amylase was found in sera from 8 patients sensitized to Blattella germanica alpha-amylase (≤0.1 kU/L). A BLAST alignment of protein sequences of Blattella germanica alpha-amylase with Aspergillus oryzae alpha amylase showed a query cover and E-value of 50% and 4E-12, respectively. An SDAP search revealed that there was a match of at least of six contiguous amino acids between the alpha-amylase of Blattella germanica and the alpha-amylase of Blomia tropicalis (Blo t 4.0101), Dermatophagoides pteronyssinus (Der p 4) and Euroglyphus maynei (Eur m 4), which are three described mite allergens.

Bottom Line: SDS-PAGE IgE-immunoblotting associated with mass spectrometry was carried out to study the cockroach IgE-binding sensitizing proteins.Pollen and cockroach were the most relevant allergen sources in the asthmatic population.Using the Component Resolved Diagnosis, it was possible to identify a new Blattella germanica cockroach allergen (Blattella alpha amylase 53 kDa) that could sensitize a relevant percentage of this population.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-331, Iran ; Laboratory of Parasitology and Allergy, Center for Research Lascaray Ikergunea, University of the Basque Country, Pº Universidad, 7, 01006 Vitoria, Spain ; Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, Pº Universidad, 7, 01006 Vitoria, Spain.

ABSTRACT

Background: There has been a significant growth in the prevalence of allergy, mainly associated to IgE-mediated disorders such as asthma and rhinitis. The identification of atopy in asthmatic patients through the measurement of specific IgE can help to identify risk factors that cause asthmatic symptoms in patients. The development and use of individualized allergen-based tests by the Component Resolved Diagnosis has been a crucial advance in the accurate diagnosis and control of allergic patients. The objective of this work was to assess the usefulness of molecular diagnosis to identify environmental allergens as possible factors influencing the development and manifestation of asthma in a group of asthmatic patients from Iran.

Studied population: 202 adult asthmatic patients treated at the Loghman Hakim Hospital and Pasteur Institute of Teheran (Iran) from 2011 to 2012. Specific IgE determined by the ImmunoCAP system were used to both evaluate the patients' atopic condition and the molecules involved in the allergic sensitization. SDS-PAGE IgE-immunoblotting associated with mass spectrometry was carried out to study the cockroach IgE-binding sensitizing proteins.

Results: Forty-five percent of all patients could be considered atopic individuals. Eighty-two percent of atopic patients were sensitized to pollen allergens. The Salsola kali (Sal k 1) and the Phleum pratense (rPhl p 1 and/or rPhl p 5) major allergens were the most common sensitizers among pollens (71% and 18%, respectively). Thirty-five percent of the atopic population was sensitized to cockroach. Four different allergens, including a previously unknown alpha-amylase, were identified in the cockroach extract. No significant associations could be demonstrated between the severity of asthma and the specific IgE levels in the atopic population. Statistical analysis identified the Sal k 1 as the main protein allergen influencing the development and expression of asthma in the studied population.

Conclusions: Pollen and cockroach were the most relevant allergen sources in the asthmatic population. The Salsola kali major allergen was the main cause for sensitization in the atopic patients suffering asthma. Using the Component Resolved Diagnosis, it was possible to identify a new Blattella germanica cockroach allergen (Blattella alpha amylase 53 kDa) that could sensitize a relevant percentage of this population.

No MeSH data available.


Related in: MedlinePlus