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Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

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ABTS•+ scavenging activity of polar extractsfrom day 7 potato wound periderm samples expressed as TEAC (μmolTrolox/g dried sample), color coded for Atlantic (red), Chipeta (green),Norkotah Russet (blue), and Yukon Gold (gold). Values are expressedas mean ± standard error of the mean (n = 6).Note that the large standard error of the mean for Yukon day 7 extractscorresponds to a percent error that is similar to the other extracts.
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fig8: ABTS•+ scavenging activity of polar extractsfrom day 7 potato wound periderm samples expressed as TEAC (μmolTrolox/g dried sample), color coded for Atlantic (red), Chipeta (green),Norkotah Russet (blue), and Yukon Gold (gold). Values are expressedas mean ± standard error of the mean (n = 6).Note that the large standard error of the mean for Yukon day 7 extractscorresponds to a percent error that is similar to the other extracts.

Mentions: As noted above, the oxidative stress associatedwith tuber wounding and the potential of potato-derived antioxidantcompounds as food preservatives3 togethermotivate quantitative assessments of such compounds in potato cultivarsamples. Polar extracts of day 3 and day 7 tissue samples were screenedfor their free radical scavenging activities. All extracts exhibitedscavenging activity, and their activities increased with incubationtime (Figures 7 and 8). Thus, it may be deduced that the cultivars contain slow- as wellas fast-acting antioxidants, underscoring the need for an assay suchas ABTS•+ that measures the scavenging capabilityof the extracts over an extended period of time.17 The assay has additional advantages such as absence ofsteric hindrance, a broad pH range, and minimal spectral interferencefrom other natural products,37,38 distinguishing thecurrent investigation from previous antioxidant research on extractsfrom potato peels.15,18,19


Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

ABTS•+ scavenging activity of polar extractsfrom day 7 potato wound periderm samples expressed as TEAC (μmolTrolox/g dried sample), color coded for Atlantic (red), Chipeta (green),Norkotah Russet (blue), and Yukon Gold (gold). Values are expressedas mean ± standard error of the mean (n = 6).Note that the large standard error of the mean for Yukon day 7 extractscorresponds to a percent error that is similar to the other extracts.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126508&req=5

fig8: ABTS•+ scavenging activity of polar extractsfrom day 7 potato wound periderm samples expressed as TEAC (μmolTrolox/g dried sample), color coded for Atlantic (red), Chipeta (green),Norkotah Russet (blue), and Yukon Gold (gold). Values are expressedas mean ± standard error of the mean (n = 6).Note that the large standard error of the mean for Yukon day 7 extractscorresponds to a percent error that is similar to the other extracts.
Mentions: As noted above, the oxidative stress associatedwith tuber wounding and the potential of potato-derived antioxidantcompounds as food preservatives3 togethermotivate quantitative assessments of such compounds in potato cultivarsamples. Polar extracts of day 3 and day 7 tissue samples were screenedfor their free radical scavenging activities. All extracts exhibitedscavenging activity, and their activities increased with incubationtime (Figures 7 and 8). Thus, it may be deduced that the cultivars contain slow- as wellas fast-acting antioxidants, underscoring the need for an assay suchas ABTS•+ that measures the scavenging capabilityof the extracts over an extended period of time.17 The assay has additional advantages such as absence ofsteric hindrance, a broad pH range, and minimal spectral interferencefrom other natural products,37,38 distinguishing thecurrent investigation from previous antioxidant research on extractsfrom potato peels.15,18,19

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

Show MeSH
Related in: MedlinePlus