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Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

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PCA score plotsfor the LC-MS data from extracts of day 3 (A) andday 7 (B) potato wound-healing samples, color coded for Atlantic (red),Chipeta (green), Norkotah Russet (blue), and Yukon Gold (gold).
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fig5: PCA score plotsfor the LC-MS data from extracts of day 3 (A) andday 7 (B) potato wound-healing samples, color coded for Atlantic (red),Chipeta (green), Norkotah Russet (blue), and Yukon Gold (gold).

Mentions: In an analogous fashion, Figure 2 and SI Figure S2 demonstrate consistencyamong LC-MSexperiments on nominally identical samples and distinct signaturesfor each of the four cultivars and wound-healing time points. Evenmore definitively than via NMR data, score plots obtained from PCAanalysis of the LC-MS experiments show polar metabolite differencesamong the clusters corresponding to Russet Norkotah, Atlantic, Chipeta,and Yukon Gold cultivars (Figure 5). As comparedwith day 3 (Figure 5A), the convergent trendof metabolite compositions at day 7 is demonstrated by both the LC-MSscore plot (Figure 5B) and the overall PCAanalysis for both healing time points (Figure 6), again aligning with the NMR-derived multivariate analysis (Figures 3 and 4). This convergenceof metabolite profiles with time, which has been observed previouslyin GC-MS-based multivariate analysis of Russet Burbank periderms,14 could reflect common biosynthetic pathways associatedwith wounding in the four cultivars. That is, if the initially distinctmetabolite pools for the four cultivars accumulate a common set ofphytochemicals associated with wound induction, these common metaboliteswill become increasingly dominant at later healing stages.


Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

PCA score plotsfor the LC-MS data from extracts of day 3 (A) andday 7 (B) potato wound-healing samples, color coded for Atlantic (red),Chipeta (green), Norkotah Russet (blue), and Yukon Gold (gold).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126508&req=5

fig5: PCA score plotsfor the LC-MS data from extracts of day 3 (A) andday 7 (B) potato wound-healing samples, color coded for Atlantic (red),Chipeta (green), Norkotah Russet (blue), and Yukon Gold (gold).
Mentions: In an analogous fashion, Figure 2 and SI Figure S2 demonstrate consistencyamong LC-MSexperiments on nominally identical samples and distinct signaturesfor each of the four cultivars and wound-healing time points. Evenmore definitively than via NMR data, score plots obtained from PCAanalysis of the LC-MS experiments show polar metabolite differencesamong the clusters corresponding to Russet Norkotah, Atlantic, Chipeta,and Yukon Gold cultivars (Figure 5). As comparedwith day 3 (Figure 5A), the convergent trendof metabolite compositions at day 7 is demonstrated by both the LC-MSscore plot (Figure 5B) and the overall PCAanalysis for both healing time points (Figure 6), again aligning with the NMR-derived multivariate analysis (Figures 3 and 4). This convergenceof metabolite profiles with time, which has been observed previouslyin GC-MS-based multivariate analysis of Russet Burbank periderms,14 could reflect common biosynthetic pathways associatedwith wounding in the four cultivars. That is, if the initially distinctmetabolite pools for the four cultivars accumulate a common set ofphytochemicals associated with wound induction, these common metaboliteswill become increasingly dominant at later healing stages.

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

Show MeSH
Related in: MedlinePlus