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Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

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PCA score plot for the overall NMR data fromextracts of the fourcultivars at both day 3 (circles) and day 7 (triangles) post wounding(W). The samples are coded for Atlantic (red, a), Chipeta (green,c), Norkotah Russet (blue, r), and Yukon Gold (gold, y).
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fig4: PCA score plot for the overall NMR data fromextracts of the fourcultivars at both day 3 (circles) and day 7 (triangles) post wounding(W). The samples are coded for Atlantic (red, a), Chipeta (green,c), Norkotah Russet (blue, r), and Yukon Gold (gold, y).

Mentions: Visual inspection of the stacked 1H NMR spectra forwound-healing tissue extracts from variouscultivars at days 3 and 7 (Figure 2 and Supporting Information (SI) Figure S1, respectively)shows good consistency among the six replicates for each cultivar,a high degree of instrumental reproducibility, and modest but cleardifferences among the four cultivars. A more rigorous multivariateanalysis of the data provides essential unbiased confirmation of thesedifferences. The PCA-associated score plots for the NMR data (Figure 3) demonstrate that the polar metabolites for eachof the four cultivars form distinct and nonoverlapping clusters, moredistinct at day 3 compared with day 7 post wounding. To compare themetabolic profiles at different wound-healing time points, PCA wascarried out for day-3 and day-7 NMR data together; the resulting scoreplot (Figure 4) shows a clear separation ofmetabolites at the two time points according to PC1. Again, a closerconvergence of the clusters for each cultivar is evident at day 7.


Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

PCA score plot for the overall NMR data fromextracts of the fourcultivars at both day 3 (circles) and day 7 (triangles) post wounding(W). The samples are coded for Atlantic (red, a), Chipeta (green,c), Norkotah Russet (blue, r), and Yukon Gold (gold, y).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126508&req=5

fig4: PCA score plot for the overall NMR data fromextracts of the fourcultivars at both day 3 (circles) and day 7 (triangles) post wounding(W). The samples are coded for Atlantic (red, a), Chipeta (green,c), Norkotah Russet (blue, r), and Yukon Gold (gold, y).
Mentions: Visual inspection of the stacked 1H NMR spectra forwound-healing tissue extracts from variouscultivars at days 3 and 7 (Figure 2 and Supporting Information (SI) Figure S1, respectively)shows good consistency among the six replicates for each cultivar,a high degree of instrumental reproducibility, and modest but cleardifferences among the four cultivars. A more rigorous multivariateanalysis of the data provides essential unbiased confirmation of thesedifferences. The PCA-associated score plots for the NMR data (Figure 3) demonstrate that the polar metabolites for eachof the four cultivars form distinct and nonoverlapping clusters, moredistinct at day 3 compared with day 7 post wounding. To compare themetabolic profiles at different wound-healing time points, PCA wascarried out for day-3 and day-7 NMR data together; the resulting scoreplot (Figure 4) shows a clear separation ofmetabolites at the two time points according to PC1. Again, a closerconvergence of the clusters for each cultivar is evident at day 7.

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

Show MeSH
Related in: MedlinePlus