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Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

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Schematic representation of the experimental design, includingtypical data and statistical multivariate analyses including PCA,OPLS-DA and S-plot analysis of liquid chromatography - mass spectrometry(LC-MS) data (total ion current (TIC) chromatograms in positive mode),leading to the detection of marker ions. Stacked LC-MS chromatogramsof the polar extracts each represent average data from six biologicalreplicates from day 3 potato wound periderm samples, color coded forNorkotah Russet (blue), Atlantic (red), Chipeta (green), and YukonGold (gold). Statistical procedures are detailed in the text.
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fig1: Schematic representation of the experimental design, includingtypical data and statistical multivariate analyses including PCA,OPLS-DA and S-plot analysis of liquid chromatography - mass spectrometry(LC-MS) data (total ion current (TIC) chromatograms in positive mode),leading to the detection of marker ions. Stacked LC-MS chromatogramsof the polar extracts each represent average data from six biologicalreplicates from day 3 potato wound periderm samples, color coded forNorkotah Russet (blue), Atlantic (red), Chipeta (green), and YukonGold (gold). Statistical procedures are detailed in the text.

Mentions: Principal component analysis(PCA) of the normalized data from NMR and MS experiments was carriedout using Simca-P+ software version 13.0 (Umetricas, Umeå,Sweden) and Pareto scaling.20 In addition,the data were subjected to orthogonal partial least-squares discriminateanalysis (OPLS-DA), in which data from a particular cultivar wereassigned to one class that was then compared with another class comprisingthe remaining cultivars. The corresponding S-plots displayed extreme“wings” that yielded chemical shifts or mass-to-charge(m/z) ratios of the biomarkers thatcontribute to compositional differences among the tissue samples (Figure 1). These variables were evaluated individually usingvariable line plots to ascertain if the markers were unique to a particularcultivar at a specified wounding time point (Figure 1).


Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.

Dastmalchi K, Cai Q, Zhou K, Huang W, Serra O, Stark RE - J. Agric. Food Chem. (2014)

Schematic representation of the experimental design, includingtypical data and statistical multivariate analyses including PCA,OPLS-DA and S-plot analysis of liquid chromatography - mass spectrometry(LC-MS) data (total ion current (TIC) chromatograms in positive mode),leading to the detection of marker ions. Stacked LC-MS chromatogramsof the polar extracts each represent average data from six biologicalreplicates from day 3 potato wound periderm samples, color coded forNorkotah Russet (blue), Atlantic (red), Chipeta (green), and YukonGold (gold). Statistical procedures are detailed in the text.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126508&req=5

fig1: Schematic representation of the experimental design, includingtypical data and statistical multivariate analyses including PCA,OPLS-DA and S-plot analysis of liquid chromatography - mass spectrometry(LC-MS) data (total ion current (TIC) chromatograms in positive mode),leading to the detection of marker ions. Stacked LC-MS chromatogramsof the polar extracts each represent average data from six biologicalreplicates from day 3 potato wound periderm samples, color coded forNorkotah Russet (blue), Atlantic (red), Chipeta (green), and YukonGold (gold). Statistical procedures are detailed in the text.
Mentions: Principal component analysis(PCA) of the normalized data from NMR and MS experiments was carriedout using Simca-P+ software version 13.0 (Umetricas, Umeå,Sweden) and Pareto scaling.20 In addition,the data were subjected to orthogonal partial least-squares discriminateanalysis (OPLS-DA), in which data from a particular cultivar wereassigned to one class that was then compared with another class comprisingthe remaining cultivars. The corresponding S-plots displayed extreme“wings” that yielded chemical shifts or mass-to-charge(m/z) ratios of the biomarkers thatcontribute to compositional differences among the tissue samples (Figure 1). These variables were evaluated individually usingvariable line plots to ascertain if the markers were unique to a particularcultivar at a specified wounding time point (Figure 1).

Bottom Line: Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions.The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids.Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, The City College of New York, City University of New York Graduate Center and Institute for Macromolecular Assemblies , New York, New York 10031, United States.

ABSTRACT
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.

Show MeSH
Related in: MedlinePlus