Limits...
Fabrication of surface plasmon resonance sensor surface with control of the non-specific adsorption and affinity for the detection of 2,4,6-trinitrotoluene using an antifouling copolymer.

Yatabe R, Onodera T, Toko K - Front Bioeng Biotechnol (2014)

Bottom Line: We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT).The detection of TNT was carried out by displacement assay with the SPR measurement.In displacement assay, the affinity between anti-TNT antibody and the sensor surface, affects to the sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Taste and Odor Sensing, Kyushu University , Fukuoka , Japan.

ABSTRACT
We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT). The hydrophilic polymer was made from mono-2-(methacryloyloxy)ethylsuccinate (MES) and 2-hydroxyethylmethacrylate (HEMA) by surface-initiated atom transfer radical polymerization. The detection of TNT was carried out by displacement assay with the SPR measurement. In displacement assay, the affinity between anti-TNT antibody and the sensor surface, affects to the sensitivity. In the SPR measurement, non-specific adsorption should be controlled because SPR sensor cannot discriminate between specific and non-specific adsorption. Therefore, the affinity and non-specific adsorption were controlled by changing the ratio of HEMA to MES. A detection limit of 0.4 ng/ml (ppb) for TNT was achieved using a sensor surface with the lowest affinity without non-specific adsorption.

No MeSH data available.


Related in: MedlinePlus

Response characteristic to TNT: calibration curves obtained by displacement assay using 25 mg/mL (25 ppm) anti-TNT antibody. The error bar shows the SD of the data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4126440&req=5

Figure 3: Response characteristic to TNT: calibration curves obtained by displacement assay using 25 mg/mL (25 ppm) anti-TNT antibody. The error bar shows the SD of the data.

Mentions: Next, Figure 3 shows the calibration curves for MES: HEMA = 1:100 and 1:1000. The points on the graph are real data and the lines on the graph are fitted curves. The error bar on each point shows the standard deviation. Table 3 shows the standard deviation of each TNT concentrations. The displacement ratio depended on the concentration of TNT for all data at MES:HEMA = 1:100. The standard deviation data had no tendency. On the other hand, the displacement ratio was independent of the TNT concentrations of 25 and 50 ng/ml (ppb) at MES:HEMA = 1:1000 as indicated by the random distribution of the data points. The standard deviation data tended to increase with the concentration of TNT. The value recorded for the 100 ng/ml sample was not reliable because of the relatively high standard deviation. Therefore, the displacement ratio only depended on the TNT concentration of 1, 5, and 10 ng/ml (ppb) at MES:HEMA = 1:1000. The data “sensor response *1” at MES:HEMA = 1:1000 was the lowest among all the conditions. This means that the amount of antibody adsorbed on the surface in the case of MES:HEMA = 1:1000 was negligible because of high kd. The response was saturated because most of the antibody had been dissociated from the surface by the high concentration of TNT. Thus, the sensor surface with MES:HEMA = 1:1000 has high sensitivity but cannot measure the concentration of TNT over a wide range. In the detection of explosives, it is more important to be able to discriminate whether or not explosives exist than to measure the concentration of the explosive compounds. Therefore, it is not necessary to be able to measure over a wide range. However, the sensor should have a sufficient measurable range to ensure the reliability of sensing in practical usage. The measurable range and the LOD can be varied by changing the MES:HEMA ratio in our sensor. Hence, the parameters can be optimized by field tests. In conclusion, the sensor surface with a small amount of MES had high sensitivity and a narrow measurable range, and the measurable range can be optimized by changing the MES:HEMA ratio if the range is required to be wider.


Fabrication of surface plasmon resonance sensor surface with control of the non-specific adsorption and affinity for the detection of 2,4,6-trinitrotoluene using an antifouling copolymer.

Yatabe R, Onodera T, Toko K - Front Bioeng Biotechnol (2014)

Response characteristic to TNT: calibration curves obtained by displacement assay using 25 mg/mL (25 ppm) anti-TNT antibody. The error bar shows the SD of the data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126440&req=5

Figure 3: Response characteristic to TNT: calibration curves obtained by displacement assay using 25 mg/mL (25 ppm) anti-TNT antibody. The error bar shows the SD of the data.
Mentions: Next, Figure 3 shows the calibration curves for MES: HEMA = 1:100 and 1:1000. The points on the graph are real data and the lines on the graph are fitted curves. The error bar on each point shows the standard deviation. Table 3 shows the standard deviation of each TNT concentrations. The displacement ratio depended on the concentration of TNT for all data at MES:HEMA = 1:100. The standard deviation data had no tendency. On the other hand, the displacement ratio was independent of the TNT concentrations of 25 and 50 ng/ml (ppb) at MES:HEMA = 1:1000 as indicated by the random distribution of the data points. The standard deviation data tended to increase with the concentration of TNT. The value recorded for the 100 ng/ml sample was not reliable because of the relatively high standard deviation. Therefore, the displacement ratio only depended on the TNT concentration of 1, 5, and 10 ng/ml (ppb) at MES:HEMA = 1:1000. The data “sensor response *1” at MES:HEMA = 1:1000 was the lowest among all the conditions. This means that the amount of antibody adsorbed on the surface in the case of MES:HEMA = 1:1000 was negligible because of high kd. The response was saturated because most of the antibody had been dissociated from the surface by the high concentration of TNT. Thus, the sensor surface with MES:HEMA = 1:1000 has high sensitivity but cannot measure the concentration of TNT over a wide range. In the detection of explosives, it is more important to be able to discriminate whether or not explosives exist than to measure the concentration of the explosive compounds. Therefore, it is not necessary to be able to measure over a wide range. However, the sensor should have a sufficient measurable range to ensure the reliability of sensing in practical usage. The measurable range and the LOD can be varied by changing the MES:HEMA ratio in our sensor. Hence, the parameters can be optimized by field tests. In conclusion, the sensor surface with a small amount of MES had high sensitivity and a narrow measurable range, and the measurable range can be optimized by changing the MES:HEMA ratio if the range is required to be wider.

Bottom Line: We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT).The detection of TNT was carried out by displacement assay with the SPR measurement.In displacement assay, the affinity between anti-TNT antibody and the sensor surface, affects to the sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Taste and Odor Sensing, Kyushu University , Fukuoka , Japan.

ABSTRACT
We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT). The hydrophilic polymer was made from mono-2-(methacryloyloxy)ethylsuccinate (MES) and 2-hydroxyethylmethacrylate (HEMA) by surface-initiated atom transfer radical polymerization. The detection of TNT was carried out by displacement assay with the SPR measurement. In displacement assay, the affinity between anti-TNT antibody and the sensor surface, affects to the sensitivity. In the SPR measurement, non-specific adsorption should be controlled because SPR sensor cannot discriminate between specific and non-specific adsorption. Therefore, the affinity and non-specific adsorption were controlled by changing the ratio of HEMA to MES. A detection limit of 0.4 ng/ml (ppb) for TNT was achieved using a sensor surface with the lowest affinity without non-specific adsorption.

No MeSH data available.


Related in: MedlinePlus