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Large-scale filament formation inhibits the activity of CTP synthetase.

Barry RM, Bitbol AF, Lorestani A, Charles EJ, Habrian CH, Hansen JM, Li HJ, Baldwin EP, Wingreen NS, Kollman JM, Gitai Z - Elife (2014)

Bottom Line: Structure-guided mutagenesis and mathematical modeling further indicate that coupling activity to polymerization promotes cooperative catalytic regulation.This previously uncharacterized regulatory mechanism is important for cellular function since a mutant that disrupts CtpS polymerization disrupts E. coli growth and metabolic regulation without reducing CTP levels.We propose that regulation by large-scale polymerization enables ultrasensitive control of enzymatic activity while storing an enzyme subpopulation in a conformationally restricted form that is readily activatable.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Princeton University, Princeton, United States.

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DON inhibition of activity does not inhibit polymerization upon CTP addition.In the converse experiment of Figure 2E, DON-inhibited CtpS incubated in substrates does not polymerize, but addition of 1 mM CTP stimulates polymerization.DOI:http://dx.doi.org/10.7554/eLife.03638.016
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fig2s5: DON inhibition of activity does not inhibit polymerization upon CTP addition.In the converse experiment of Figure 2E, DON-inhibited CtpS incubated in substrates does not polymerize, but addition of 1 mM CTP stimulates polymerization.DOI:http://dx.doi.org/10.7554/eLife.03638.016


Large-scale filament formation inhibits the activity of CTP synthetase.

Barry RM, Bitbol AF, Lorestani A, Charles EJ, Habrian CH, Hansen JM, Li HJ, Baldwin EP, Wingreen NS, Kollman JM, Gitai Z - Elife (2014)

DON inhibition of activity does not inhibit polymerization upon CTP addition.In the converse experiment of Figure 2E, DON-inhibited CtpS incubated in substrates does not polymerize, but addition of 1 mM CTP stimulates polymerization.DOI:http://dx.doi.org/10.7554/eLife.03638.016
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126345&req=5

fig2s5: DON inhibition of activity does not inhibit polymerization upon CTP addition.In the converse experiment of Figure 2E, DON-inhibited CtpS incubated in substrates does not polymerize, but addition of 1 mM CTP stimulates polymerization.DOI:http://dx.doi.org/10.7554/eLife.03638.016
Bottom Line: Structure-guided mutagenesis and mathematical modeling further indicate that coupling activity to polymerization promotes cooperative catalytic regulation.This previously uncharacterized regulatory mechanism is important for cellular function since a mutant that disrupts CtpS polymerization disrupts E. coli growth and metabolic regulation without reducing CTP levels.We propose that regulation by large-scale polymerization enables ultrasensitive control of enzymatic activity while storing an enzyme subpopulation in a conformationally restricted form that is readily activatable.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Princeton University, Princeton, United States.

Show MeSH