Large-scale filament formation inhibits the activity of CTP synthetase.
Bottom Line: Structure-guided mutagenesis and mathematical modeling further indicate that coupling activity to polymerization promotes cooperative catalytic regulation.This previously uncharacterized regulatory mechanism is important for cellular function since a mutant that disrupts CtpS polymerization disrupts E. coli growth and metabolic regulation without reducing CTP levels.We propose that regulation by large-scale polymerization enables ultrasensitive control of enzymatic activity while storing an enzyme subpopulation in a conformationally restricted form that is readily activatable.
Affiliation: Department of Molecular Biology, Princeton University, Princeton, United States.Show MeSH
Related in: MedlinePlus
Mentions: D) We have demonstrated CtpS disassembly in the experiment of Figure 1C by including a supplemental figure showing the corresponding light scattering change over time (Figure 1–figure supplement 4).
Affiliation: Department of Molecular Biology, Princeton University, Princeton, United States.